• Western blot analysis of extracts of various cell lines, using GIPR antibody (STJ111858) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using GIPR Rabbit polyclonal antibody (STJ111858) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using GIPR rabbit polyclonal antibody (STJ111858) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of various cell lines, using GIPR antibody (STJ111858) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-GIPR antibody (22-138) (STJ111858)

SKU:
STJ111858

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GIPR (22-138) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GIPR
Gene ID: 2696
Uniprot ID: GIPR_HUMAN
Immunogen Region: 22-138
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-138 of human GIPR (NP_000155.1).
Immunogen Sequence: RAETGSKGQTAGELYQRWER YRRECQETLAAAEPPSGLAC NGSFDMYVCWDYAAPNATAR ASCPWYLPWHHHVAAGFVLR QCGSDGQWGLWRDHTQCENP EKNEAFLDQRLILERLQ
Post Translational Modifications N-glycosylation is required for cell surface expression and lengthens receptor half-life by preventing degradation in the ER.
Function This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Protein Name Gastric Inhibitory Polypeptide Receptor
Gip-R
Glucose-Dependent Insulinotropic Polypeptide Receptor
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-420092
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Gastric Inhibitory Polypeptide Receptor antibody
Anti-Gip-R antibody
Anti-Glucose-Dependent Insulinotropic Polypeptide Receptor antibody
Anti-GIPR antibody

Information sourced from Uniprot.org

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