| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GET4 (1-327) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GET4 |
| Gene ID: |
51608 |
| Uniprot ID: |
GET4_HUMAN |
| Immunogen Region: |
1-327 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human GET4 (NP_057033.2). |
| Immunogen Sequence: |
MAAAAAMAEQESARNGGRNR GGVQRVEGKLRASVEKGDYY EAHQMYRTLFFRYMSQSKHT EARELMYSGALLFFSHGQQN SAADLSMLVLESLEKAEVEV ADELLENLAKVFSLMDPNSP ERVTFVSRALKWSSGGSGKL GHPRLHQLLALTLWKEQNYC ESRYHFLHSADGEGCANMLV EYSTSRGFRSEVDMFVAQAV LQFLCLKNKSSASVVFTTYT QKHPSIEDGPPFVEPLLNF |
| Post Translational Modifications | Ubiquitinated by RNF12, leading to proteasomal degradation. When unassembled from BAG6.ubiquitinylation is modulated by BAG6 quality control role and effectuated by RNF126. |
| Function | As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates in their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation. The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum. Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome. Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome. |
| Protein Name | Golgi To Er Traffic Protein 4 HomologConserved Edge-Expressed ProteinTransmembrane Domain Recognition Complex 35 Kda SubunitTrc35 |
| Database Links | Reactome: R-HSA-9609523 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Golgi To Er Traffic Protein 4 Homolog antibodyAnti-Conserved Edge-Expressed Protein antibodyAnti-Transmembrane Domain Recognition Complex 35 Kda Subunit antibodyAnti-Trc35 antibodyAnti-GET4 antibodyAnti-C7orf20 antibodyAnti-CEE antibodyAnti-TRC35 antibodyAnti-CGI-20 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
