| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GEMIN2 (180-280) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GEMIN2 |
| Gene ID: |
8487 |
| Uniprot ID: |
GEMI2_HUMAN |
| Immunogen Region: |
180-280 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 180-280 of human Gemin 2 (NP_003607.1) |
| Immunogen Sequence: |
LSIVSRMNQATVTSVLEYLS NWFGERDFTPELGRWLYALL ACLEKPLLPEAHSLIRQLAR RCSEVRLLVDSKDDERVPAL NLLICLVSRYFDQRDLADEP S |
| Function | The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound. |
| Protein Name | Gem-Associated Protein 2Gemin-2Component Of Gems 2Survival Of Motor Neuron Protein-Interacting Protein 1Smn-Interacting Protein 1 |
| Database Links | Reactome: R-HSA-191859Reactome: R-HSA-9754678 |
| Cellular Localisation | NucleusGemCytoplasmLocalized In Subnuclear Structures Next To Coiled BodiesCalled GemsWhich Are Highly Enriched In Spliceosomal SnrnpsAlso Found In The Cytoplasm |
| Alternative Antibody Names | Anti-Gem-Associated Protein 2 antibodyAnti-Gemin-2 antibodyAnti-Component Of Gems 2 antibodyAnti-Survival Of Motor Neuron Protein-Interacting Protein 1 antibodyAnti-Smn-Interacting Protein 1 antibodyAnti-GEMIN2 antibodyAnti-SIP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
