| Host: | Goat |
| Applications: | Pep-ELISA/WB/FC |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Gelsolin/GSN (Internal) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 90kDa observed in Human Colorectal cancer lysates and in prelominary testing of Human Spleen and Parathyroid lysate (calculated MW of 85.7kDa according to NP_000168.1). Recomme |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | GSN |
| Gene ID: | 2934 |
| Uniprot ID: | GELS_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000168.1; NP_937895.1; NP_001121138.1; NP_001244958.1; NP_001244959.1; NP_001121135.2; NP_001340005.1; NP_001340007.1 |
| Specificity: | This antibody is expected to recognize isoforms a, b, c, d, e, f, g, h |
| Immunogen Sequence: | PRLKDKKMDAHP |
| Post Translational Modifications | Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids. |
| Function | Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. |
| Protein Name | GelsolinAgelActin-Depolymerizing FactorAdfBrevin |
| Database Links | Reactome: R-HSA-264870Reactome: R-HSA-6798695Reactome: R-HSA-9662361Reactome: R-HSA-977225 |
| Cellular Localisation | Isoform 2: CytoplasmCytoskeletonIsoform 1: Secreted |
| Alternative Antibody Names | Anti-Gelsolin antibodyAnti-Agel antibodyAnti-Actin-Depolymerizing Factor antibodyAnti-Adf antibodyAnti-Brevin antibodyAnti-GSN antibody |
Information sourced from Uniprot.org
