• Western blot analysis of extracts of various cell lines, using GDNF antibody (STJ116846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of SH-SY5Y cells using GDNF rabbit polyclonal antibody (STJ116846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-87MG cells using GDNF rabbit polyclonal antibody (STJ116846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GDNF antibody (109-211) (STJ116846)

SKU:
STJ116846

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GDNF (109-211) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GDNF
Gene ID: 2668
Uniprot ID: GDNF_HUMAN
Immunogen Region: 109-211
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 109-211 of human GDNF (NP_000505.1).
Immunogen Sequence: RGQRGKNRGCVLTAIHLNVT DLGLGYETKEELIFRYCSGS CDAAETTYDKILKNLSRNRR LVSDKVGQACCRPIAFDDDL SFLDDNLVYHILRKHSAKRC GCI
Tissue Specificity In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.
Function Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Protein Name Glial Cell Line-Derived Neurotrophic Factor
Hgdnf
Astrocyte-Derived Trophic Factor
Atf
Database Links Reactome: R-HSA-419037
Reactome: R-HSA-5673001
Reactome: R-HSA-8853659
Cellular Localisation Secreted
Alternative Antibody Names Anti-Glial Cell Line-Derived Neurotrophic Factor antibody
Anti-Hgdnf antibody
Anti-Astrocyte-Derived Trophic Factor antibody
Anti-Atf antibody
Anti-GDNF antibody

Information sourced from Uniprot.org

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