• Western blot analysis of extracts of various cell lines, using GDNF antibody (STJ116846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of SH-SY5Y cells using GDNF rabbit polyclonal antibody (STJ116846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-87MG cells using GDNF rabbit polyclonal antibody (STJ116846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GDNF antibody (109-211) (STJ116846)

SKU:
STJ116846

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GDNF
Gene ID: 2668
Uniprot ID: GDNF_HUMAN
Immunogen Region: 109-211
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 109-211 of human GDNF (NP_000505.1).
Immunogen Sequence: RGQRGKNRGCVLTAIHLNVT DLGLGYETKEELIFRYCSGS CDAAETTYDKILKNLSRNRR LVSDKVGQACCRPIAFDDDL SFLDDNLVYHILRKHSAKRC GCI
Tissue Specificity In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.
Function Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor. Involved in the development of the neural crest.
Protein Name Glial Cell Line-Derived Neurotrophic Factor
Hgdnf
Astrocyte-Derived Trophic Factor
Atf
Database Links Reactome: R-HSA-419037
Reactome: R-HSA-5673001
Reactome: R-HSA-8853659
Reactome: R-HSA-9830674
Cellular Localisation Secreted
Alternative Antibody Names Anti-Glial Cell Line-Derived Neurotrophic Factor antibody
Anti-Hgdnf antibody
Anti-Astrocyte-Derived Trophic Factor antibody
Anti-Atf antibody
Anti-GDNF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance