• Confocal immunofluorescence analysis of U2OS cells using GCSH antibody (STJ115650) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using GCSH Rabbit polyclonal antibody (STJ115650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using GCSH Rabbit polyclonal antibody (STJ115650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GCSH Rabbit polyclonal antibody (STJ115650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using GCSH Rabbit polyclonal antibody (STJ115650) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GCSH antibody (1-173) (STJ115650)

SKU:
STJ115650

£112.50 - £524.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCSH
Gene ID: 2653
Uniprot ID: GCSH_HUMAN
Immunogen Region: 1-173
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human GCSH (NP_004474.2).
Immunogen Sequence: MALRVVRSVRALLCTLRAVP SPAAPCPPRPWQLGVGAVRT LRTGPALLSVRKFTEKHEWV TTENGIGTVGISNFAQEALG DVVYCSLPEVGTKLNKQDEF GALESVKAASELYSPLSGEV TEINEALAENPGLVNKSCYE DGWLIKMTLSNPSELDELMS EEAYEKYIKSIEE
Function The glycine cleavage system catalyzes the degradation of glycine. The H protein (GCSH) shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST). Has a pivotal role in the lipoylation of enzymes involved in cellular energetics such as the mitochondrial dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (DLAT), and the mitochondrial dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (DLST).
Protein Name Glycine Cleavage System H Protein - Mitochondrial
Lipoic Acid-Containing Protein
Database Links Reactome: R-HSA-6783984
Reactome: R-HSA-9857492
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Glycine Cleavage System H Protein - Mitochondrial antibody
Anti-Lipoic Acid-Containing Protein antibody
Anti-GCSH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance