• Western blot analysis of various lysates, using GCLC rabbit polyclonal antibody (STJ114916) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of Jurkat cells using GCLC rabbit polyclonal antibody (STJ114916) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using GCLC rabbit polyclonal antibody (STJ114916) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-GCLC antibody (1-252) (STJ114916)

SKU:
STJ114916

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GCLC (1-252) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:3000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCLC
Gene ID: 2729
Uniprot ID: GSH1_HUMAN
Immunogen Region: 1-252
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human GCLC (NP_001489.1).
Immunogen Sequence: MGLLSQGSPLSWEETKRHAD HVRRHGILQFLHIYHAVKDR HKDVLKWGDEVEYMLVSFDH ENKKVRLVLSGEKVLETLQE KGERTNPNHPTLWRPEYGSY MIEGTPGQPYGGTMSEFNTV EANMRKRRKEATSILEENQA LCTITSFPRLGCPGFTLPEV KPNPVEGGASKSLFFPDEAI NKHPRFSTLTRNIRHRRGEK VVINVPIFKDKNTPSPFIET FTEDDEASRASKPDHIYMD
Function Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.
Protein Name Glutamate--Cysteine Ligase Catalytic Subunit
Gcs Heavy Chain
Gamma-Ecs
Gamma-Glutamylcysteine Synthetase
Database Links Reactome: R-HSA-174403
Reactome: R-HSA-5578999
Reactome: R-HSA-9818027
Alternative Antibody Names Anti-Glutamate--Cysteine Ligase Catalytic Subunit antibody
Anti-Gcs Heavy Chain antibody
Anti-Gamma-Ecs antibody
Anti-Gamma-Glutamylcysteine Synthetase antibody
Anti-GCLC antibody
Anti-GLCL antibody
Anti-GLCLC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance