| Short Description : | Rabbit polyclonal GCLC (1-252) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:3000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GCLC |
| Gene ID: | 2729 |
| Uniprot ID: | GSH1_HUMAN |
| Immunogen Region: | 1-252 |
| Immunogen Sequence: | MGLLSQGSPLSWEETKRHAD HVRRHGILQFLHIYHAVKDR HKDVLKWGDEVEYMLVSFDH ENKKVRLVLSGEKVLETLQE KGERTNPNHPTLWRPEYGSY MIEGTPGQPYGGTMSEFNTV EANMRKRRKEATSILEENQA LCTITSFPRLGCPGFTLPEV KPNPVEGGASKSLFFPDEAI NKHPRFSTLTRNIRHRRGEK VVINVPIFKDKNTPSPFIET FTEDDEASRASKPDHIYMD |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human GCLC (NP_001489.1). |
| Function | Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis. |
| Protein Name | Glutamate--Cysteine Ligase Catalytic SubunitGcs Heavy ChainGamma-EcsGamma-Glutamylcysteine Synthetase |
| Database Links | Reactome: R-HSA-174403Reactome: R-HSA-5578999Reactome: R-HSA-9818027 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Glutamate--Cysteine Ligase Catalytic Subunit antibodyAnti-Gcs Heavy Chain antibodyAnti-Gamma-Ecs antibodyAnti-Gamma-Glutamylcysteine Synthetase antibodyAnti-GCLC antibodyAnti-GLCL antibodyAnti-GLCLC antibody |
Information sourced from Uniprot.org
