Anti-GCLC antibody (1-252) (STJ114916)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GCLC (1-252) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:3000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GCLC |
| Gene ID | 2729 |
| Uniprot ID | GSH1_HUMAN |
| Immunogen Region | 1-252 |
| Immunogen Sequence | MGLLSQGSPLSWEETKRHAD HVRRHGILQFLHIYHAVKDR HKDVLKWGDEVEYMLVSFDH ENKKVRLVLSGEKVLETLQE KGERTNPNHPTLWRPEYGSY MIEGTPGQPYGGTMSEFNTV EANMRKRRKEATSILEENQA LCTITSFPRLGCPGFTLPEV KPNPVEGGASKSLFFPDEAI NKHPRFSTLTRNIRHRRGEK VVINVPIFKDKNTPSPFIET FTEDDEASRASKPDHIYMD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human GCLC (NP_001489.1). |
Additional Info
| Function | Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis. |
| Protein Name | Glutamate--Cysteine Ligase Catalytic SubunitGcs Heavy ChainGamma-EcsGamma-Glutamylcysteine Synthetase |
| Database Links | Reactome: R-HSA-174403Reactome: R-HSA-5578999Reactome: R-HSA-9818027 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Glutamate--Cysteine Ligase Catalytic Subunit antibodyAnti-Gcs Heavy Chain antibodyAnti-Gamma-Ecs antibodyAnti-Gamma-Glutamylcysteine Synthetase antibodyAnti-GCLC antibodyAnti-GLCL antibodyAnti-GLCLC antibody |
Information sourced from Uniprot.org