Western blot analysis of lysates from Rat liver using GCKR Rabbit polyclonal antibody (STJ27645) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitExposure time: 90 s.

Anti-GCKR antibody (1-300) (STJ27645)

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STJ27645
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCKR
Gene ID: 2646
Uniprot ID: GCKR_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCKR (NP_001477.2).
Immunogen Sequence: MPGTKRFQHVIETPEPGKWE LSGYEAAVPITEKSNPLTQD LDKADAENIVRLLGQCDAEI FQEEGQALSTYQRLYSESIL TTMVQVAGKVQEVLKEPDGG LVVLSGGGTSGRMAFLMSVS FNQLMKGLGQKPLYTYLIAG GDRSVVASREGTEDSALHGI EELKKVAAGKKRVIVIGISV GLSAPFVAGQMDCCMNNTAV FLPVLVGFNPVSMARNDPIE DWSSTFRQVAERMQKMQEK
Tissue Specificity Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
Function Regulates glucokinase (GCK) by forming an inactive complex with this enzyme. Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.
Protein Name Glucokinase Regulatory Protein
Gkrp
Glucokinase Regulator
Database Links Reactome: R-HSA-170822
Reactome: R-HSA-5619107
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Under Low Glucose Concentrations
Gckr Associates With Gck And The Inactive Complex Is Recruited To The Hepatocyte Nucleus
Alternative Antibody Names Anti-Glucokinase Regulatory Protein antibody
Anti-Gkrp antibody
Anti-Glucokinase Regulator antibody
Anti-GCKR antibody

Information sourced from Uniprot.org

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