Anti-GCK antibody (250-465) (STJ117253)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GCK (250-465) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000 |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GCK |
| Gene ID | 2645 |
| Uniprot ID | HXK4_HUMAN |
| Immunogen Region | 250-465 |
| Immunogen Sequence | RMCVNTEWGAFGDSGELDEF LLEYDRLVDESSANPGQQLY EKLIGGKYMGELVRLVLLRL VDENLLFHGEASEQLRTRGA FETRFVSQVESDTGDRKQIY NILSTLGLRPSTTDCDIVRR ACESVSTRAAHMCSAGLAGV INRMRESRSEDVMRITVGVD GSVYKLHPSFKERFHASVRR LTPSCEITFIESEEGSGRGA ALVSAVACKKACMLGQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human Glucokinase (Glucokinase (GCK) ) (NP_000153.1). |
Additional Info
| Function | Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion. In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. Required to provide D-glucose 6-phosphate for the synthesis of glycogen. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. |
| Protein Name | Hexokinase-4Hk4GlucokinaseHexokinase Type IvHk IvHexokinase-D |
| Database Links | Reactome: R-HSA-170822Reactome: R-HSA-210745Reactome: R-HSA-5619073Reactome: R-HSA-5619107Reactome: R-HSA-70171Reactome: R-HSA-9615017 |
| Cellular Localisation | CytoplasmNucleusMitochondrionUnder Low Glucose ConcentrationsGck Associates With Gckr And The Inactive Complex Is Recruited To The Hepatocyte Nucleus |
| Alternative Antibody Names | Anti-Hexokinase-4 antibodyAnti-Hk4 antibodyAnti-Glucokinase antibodyAnti-Hexokinase Type Iv antibodyAnti-Hk Iv antibodyAnti-Hexokinase-D antibodyAnti-GCK antibody |
Information sourced from Uniprot.org