| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GCH1 |
| Gene ID: | 2643 |
| Uniprot ID: | GCH1_HUMAN |
| Immunogen Region: | 55-250 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 55-250 of human GCH1 (NP_000152.1). |
| Immunogen Sequence: | GERPRSEEDNELNLPNLAAA YSSILSSLGENPQRQGLLKT PWRAASAMQFFTKGYQETIS DVLNDAIFDEDHDEMVIVKD IDMFSMCEHHLVPFVGKVHI GYLPNKQVLGLSKLARIVEI YSRRLQVQERLTKQIAVAIT EALRPAGVGVVVEATHMCMV MRGVQKMNSKTVTSTMLGVF REDPKTREEFLTLIRS |
| Tissue Specificity | In epidermis, expressed predominantly in basal undifferentiated keratinocytes and in some but not all melanocytes (at protein level). |
| Post Translational Modifications | Phosphorylated by casein kinase II at Ser-81 in HAECs during oscillatory shear stress.phosphorylation at Ser-81 results in increased enzyme activity. |
| Function | Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown. |
| Protein Name | Gtp Cyclohydrolase 1Gtp Cyclohydrolase IGtp-Ch-I |
| Database Links | Reactome: R-HSA-1474151 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Gtp Cyclohydrolase 1 antibodyAnti-Gtp Cyclohydrolase I antibodyAnti-Gtp-Ch-I antibodyAnti-GCH1 antibodyAnti-DYT5 antibodyAnti-GCH antibody |
Information sourced from Uniprot.org