• Immunofluorescence analysis of PC-12 cells using GCH1 Rabbit polyclonal antibody (STJ118007) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using GCH1 antibody (STJ118007) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-GCH1 antibody (55-250) (STJ118007)

SKU:
STJ118007

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCH1
Gene ID: 2643
Uniprot ID: GCH1_HUMAN
Immunogen Region: 55-250
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 55-250 of human GCH1 (NP_000152.1).
Immunogen Sequence: GERPRSEEDNELNLPNLAAA YSSILSSLGENPQRQGLLKT PWRAASAMQFFTKGYQETIS DVLNDAIFDEDHDEMVIVKD IDMFSMCEHHLVPFVGKVHI GYLPNKQVLGLSKLARIVEI YSRRLQVQERLTKQIAVAIT EALRPAGVGVVVEATHMCMV MRGVQKMNSKTVTSTMLGVF REDPKTREEFLTLIRS
Tissue Specificity In epidermis, expressed predominantly in basal undifferentiated keratinocytes and in some but not all melanocytes (at protein level).
Post Translational Modifications Phosphorylated by casein kinase II at Ser-81 in HAECs during oscillatory shear stress.phosphorylation at Ser-81 results in increased enzyme activity.
Function Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown.
Protein Name Gtp Cyclohydrolase 1
Gtp Cyclohydrolase I
Gtp-Ch-I
Database Links Reactome: R-HSA-1474151
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Gtp Cyclohydrolase 1 antibody
Anti-Gtp Cyclohydrolase I antibody
Anti-Gtp-Ch-I antibody
Anti-GCH1 antibody
Anti-DYT5 antibody
Anti-GCH antibody

Information sourced from Uniprot.org

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