• Confocal imaging of paraffin-embedded Mouse brain tissue using GAP43 Rabbit monoclonal antibody (STJ11101593, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Mouse eye tissue using GAP43 Rabbit monoclonal antibody (STJ11101593, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat brain tissue using GAP43 Rabbit monoclonal antibody (STJ11101593, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat eye tissue using GAP43 Rabbit monoclonal antibody (STJ11101593, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using GAP43 Rabbit monoclonal antibody (STJ11101593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GAP43 antibody (139-238) [S3MR] (STJ11101593)

SKU:
STJ11101593

€183.13 - €799.38
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GAP43 (139-238) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GAP43
Gene ID: 2596
Uniprot ID: NEUM_HUMAN
Immunogen Region: 139-238
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 139-238 of human GAP43 (P17677).
Immunogen Sequence: ETESATKASTDNSPSSKAED APAKEEPKQADVPAAVTAAA ATTPAAEDAAAKATAQPPTE TGESSQAEENIEAVDETKPK ESARQDEGKEEEPEADQEHA
Post Translational Modifications Phosphorylated. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylated by ZDHHC3. Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.
Function This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Protein Name Neuromodulin
Axonal Membrane Protein Gap-43
Growth-Associated Protein 43
Neural Phosphoprotein B-50
Pp46
Database Links Reactome: R-HSA-373760
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Growth Cone Membrane
Synapse
Filopodium Membrane
Perikaryon
Dendrite
Axon
Cytoplasm
Cytoplasmic Surface Of Growth Cone And Synaptic Plasma Membranes
Alternative Antibody Names Anti-Neuromodulin antibody
Anti-Axonal Membrane Protein Gap-43 antibody
Anti-Growth-Associated Protein 43 antibody
Anti-Neural Phosphoprotein B-50 antibody
Anti-Pp46 antibody
Anti-GAP43 antibody

Information sourced from Uniprot.org

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