• Immunofluorescence analysis of rat brain cells using GAD65/GAD2 Rabbit polyclonal antibody (STJ23739) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GAD65/GAD2 antibody (STJ23739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GAD2 antibody (401-585) (STJ23739)

SKU:
STJ23739

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GAD2 (401-585) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GAD2
Gene ID: 2572
Uniprot ID: DCE2_HUMAN
Immunogen Region: 401-585
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 401-585 of human GAD65/GAD2 (NP_001127838.1).
Immunogen Sequence: PLQCSALLVREEGLMQNCNQ MHASYLFQQDKHYDLSYDTG DKALQCGRHVDVFKLWLMWR AKGTTGFEAHVDKCLELAEY LYNIIKNREGYEMVFDGKPQ HTNVCFWYIPPSLRTLEDNE ERMSRLSKVAPVIKARMMEY GTTMVSYQPLGDKVNFFRMV ISNPAATHQDIDFLIEEIER LGQDL
Post Translational Modifications Phosphorylated.which does not affect kinetic parameters or subcellular location. Palmitoylated.which is required for presynaptic clustering.
Function Catalyzes the production of GABA.
Protein Name Glutamate Decarboxylase 2
65 Kda Glutamic Acid Decarboxylase
Gad-65
Glutamate Decarboxylase 65 Kda Isoform
Database Links Reactome: R-HSA-888568
Reactome: R-HSA-888590
Reactome: R-HSA-9022927
Cellular Localisation Cytoplasm
Cytosol
Cytoplasmic Vesicle
Presynaptic Cell Membrane
Lipid-Anchor
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Associated To Cytoplasmic Vesicles
In Neurons
Cytosolic Leaflet Of Golgi Membranes And Presynaptic Clusters
Alternative Antibody Names Anti-Glutamate Decarboxylase 2 antibody
Anti-65 Kda Glutamic Acid Decarboxylase antibody
Anti-Gad-65 antibody
Anti-Glutamate Decarboxylase 65 Kda Isoform antibody
Anti-GAD2 antibody
Anti-GAD65 antibody

Information sourced from Uniprot.org

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