Anti-GABRG2 antibody (Cytoplasmic Loop) (STJA0003844)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenFusion protein from the cytoplasmic loop of the gamma 2 subunit
General Information
| Short Description | Rabbit polyclonal anti-GABAA Receptor g2 (Cytoplasmic Loop) for use in WB and IHC in Mouse, Rat, Bovine, Canine, Chicken, Human and Non-Human Primates samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:1000IHC 1:300 |
| Formulation | 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Gabrg2 |
| Gene ID | 29709 |
| Uniprot ID | GBRG2_RAT |
| Immunogen | Fusion protein from the cytoplasmic loop of the gamma 2 subunit |
| Immunogen Region | Cytoplasmic Loop |
Additional Info
| Post Translational Modifications | Palmitoylated by ZDHHC3/GODZ.required for the accumulation of GABA(A) receptors at the postsynaptic membrane of inhibitory GABAergic synapses. Glycosylated. |
| Function | Gamma subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Gamma-2/GABRG2-containing GABAARs are found at both synaptic and extrasynaptic sites. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity.the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation. Extrasynaptic gamma-2-containing receptors contribute to the tonic GABAergic inhibition. GABAARs function also as histamine receptor where histamine binds at the interface of two neighboring beta subunits and potentiates GABA response in a gamma-2 subunit-controlled manner. |
| Protein Name | Gamma-Aminobutyric Acid Receptor Subunit Gamma-2Gaba(A Receptor Subunit Gamma-2Gabaar Subunit Gamma-2 |
| Database Links | Reactome: R-RNO-977443 |
| Cellular Localisation | Postsynaptic Cell MembraneMulti-Pass Membrane ProteinCell MembraneCell ProjectionDendriteCytoplasmic Vesicle MembraneThe Gamma-2 Subunits Are Present In Golgi Synapses And On The Extrasynaptic Membranes (When Associated With Alpha-1/6 And Beta-2/3 Subunits)And In Some Of The Mossy Fiber To Granule Cell Synapses (When Associated With Alpha-6 And Beta-2/3 Subunits)Present In Gabaergic And Glutamatergic Synapses On Granule Cells |
| Alternative Antibody Names | Anti-Gamma-Aminobutyric Acid Receptor Subunit Gamma-2 antibodyAnti-Gaba(A Receptor Subunit Gamma-2 antibodyAnti-Gabaar Subunit Gamma-2 antibodyAnti-Gabrg2 antibody |
Information sourced from Uniprot.org