| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Mouse/Rat/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-GABRG2 (400-410) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 50kDa band observed in Mouse and Rat Brain lysates (calculated MW of 55.1kDa according to Mouse NP_032099.1 and 54.1kDa according to Rat NP_899156.1). Recommended concentration: |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
GABRG2 |
| Gene ID: |
2566 |
| Uniprot ID: |
GBRG2_HUMAN |
| Immunogen Region: |
400-410 |
| Accession Number: |
NP_944494.1; NP_000807.2; NP_944493.2 |
| Specificity: |
This antibody is expected to recognize all reported isoforms: NP_944494.1; NP_000807.2; NP_944493.2. Note the aa numbers used in the name are from NP_944494.1. |
| Immunogen Sequence: |
NNATHLQERDE |
| Function | Gamma subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Gamma-2/GABRG2-containing GABAARs are found at both synaptic and extrasynaptic sites. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity.the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation. Extrasynaptic gamma-2-containing receptors contribute to the tonic GABAergic inhibition. GABAARs function also as histamine receptor where histamine binds at the interface of two neighboring beta subunits and potentiates GABA response in a gamma-2 subunit-controlled manner. |
| Protein Name | Gamma-Aminobutyric Acid Receptor Subunit Gamma-2Gaba(A Receptor Subunit Gamma-2Gabaar Subunit Gamma-2 |
| Database Links | Reactome: R-HSA-1236394Reactome: R-HSA-977443 |
| Cellular Localisation | Postsynaptic Cell MembraneMulti-Pass Membrane ProteinCell MembraneCell ProjectionDendriteCytoplasmic Vesicle Membrane |
| Alternative Antibody Names | Anti-Gamma-Aminobutyric Acid Receptor Subunit Gamma-2 antibodyAnti-Gaba(A Receptor Subunit Gamma-2 antibodyAnti-Gabaar Subunit Gamma-2 antibodyAnti-GABRG2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
