| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-GABRA4 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 70kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 61.6kDa according to NP_000800.2). Recommended concentration: 0.1-0.3µg/ml.IHC |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
GABRA4 |
| Gene ID: |
2557 |
| Uniprot ID: |
GBRA4_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_000800.2 |
| Immunogen Sequence: |
EKAKRKTSKPPQE |
| Function | Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. GABAARs containing alpha-4 are predominantly extrasynaptic, contributing to tonic inhibition in dentate granule cells and thalamic relay neurons. Extrasynaptic alpha-4-containing GABAARs control levels of excitability and network activity. GABAAR containing alpha-4-beta-3-delta subunits can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness. |
| Protein Name | Gamma-Aminobutyric Acid Receptor Subunit Alpha-4Gaba(A Receptor Subunit Alpha-4Gabaar Subunit Alpha-4 |
| Database Links | Reactome: R-HSA-977443 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinPostsynaptic Cell Membrane |
| Alternative Antibody Names | Anti-Gamma-Aminobutyric Acid Receptor Subunit Alpha-4 antibodyAnti-Gaba(A Receptor Subunit Alpha-4 antibodyAnti-Gabaar Subunit Alpha-4 antibodyAnti-GABRA4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
