• Western blot of mouse brain lysates from wild type (WT) and Alpha 2-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa Alpha 2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from Alpha 2-knockout animals.
  • Western blot of rat cortical neurons showing specific immunolabeling of the ~51 kDa Alpha 2-subunit of the GABAA-R (1:1000). Image kindly provided by Lidong Liu, University of British Columbia, Vancouver.

Anti-GABRA2 antibody (Cytoplasmic Loop) (STJA0003641)

SKU:
STJA0003641-100

£454.50
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Host: Rabbit
Applications: WB/ICC
Reactivity: Mouse/Bovine/Canine/Human/Non-Human Primates/Rat/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GABA A Receptor a 2 (Cytoplasmic Loop) is suitable for use in Western Blot and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
ICC 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Gabra2
Uniprot ID: GBRA2_RAT
Immunogen Region: Cytoplasmic Loop
Immunogen: Fusion protein from the cytoplasmic loop of the alpha 2 subunit.
Tissue Specificity Expressed in brain (at protein level).
Post Translational Modifications Glycosylated.
Function Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. The alpha-2 subunit exhibits synaptogenic activity together with beta-2 and very little to no activity together with beta-3, the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation.
Protein Name Gamma-Aminobutyric Acid Receptor Subunit Alpha-2
Gaba(A Receptor Subunit Alpha-2
Gabaar Subunit Alpha-2
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Cytoplasmic Vesicle Membrane
Cell Projection
Dendrite
Alternative Antibody Names Anti-Gamma-Aminobutyric Acid Receptor Subunit Alpha-2 antibody
Anti-Gaba(A Receptor Subunit Alpha-2 antibody
Anti-Gabaar Subunit Alpha-2 antibody
Anti-Gabra2 antibody
Anti-Gabra-2 antibody

Information sourced from Uniprot.org

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