• Immunofluorescence analysis of paraffin-embedded Human liver tissue using FMO1 Rabbit polyclonal antibody (STJ11105610) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of lysates from Rat liver using FMO1 Rabbit polyclonal antibody (STJ11105610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-FMO1 antibody (371-470) (STJ11105610)

SKU:
STJ11105610

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Flavin-Containing Monooxygenase 1 (371-470) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FMO1
Gene ID: 2326
Uniprot ID: FMO1_HUMAN
Immunogen Region: 371-470
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 371-470 of human FMO1 (NP_002012.1).
Immunogen Sequence: LIKPLGSMIPTGETQARWAV RVLKGVNKLPPPSVMIEEIN ARKENKPSWFGLCYCKALQS DYITYIDELLTYINAKPNLF SMLLTDPHLALTVFFGPCSP
Tissue Specificity Expressed mainly in fetal and adult liver.
Function Broad spectrum monooxygenase that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including xenobiotics. Catalyzes the S-oxygenation of hypotaurine to produce taurine, an organic osmolyte involved in cell volume regulation as well as a variety of cytoprotective and developmental processes. In vitro, catalyzes the N-oxygenation of trimethylamine (TMA) to produce trimethylamine N-oxide (TMAO) and could therefore participate to the detoxification of this compound that is generated by the action of gut microbiota from dietary precursors such as choline, choline containing compounds, betaine or L-carnitine.
Protein Name Flavin-Containing Monooxygenase 1
Dimethylaniline Monooxygenase N-Oxide-Forming 1
Dimethylaniline Oxidase 1
Fetal Hepatic Flavin-Containing Monooxygenase 1
Fmo 1
Trimethylamine Monooxygenase
Database Links Reactome: R-HSA-1614558
Reactome: R-HSA-217271
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Flavin-Containing Monooxygenase 1 antibody
Anti-Dimethylaniline Monooxygenase N-Oxide-Forming 1 antibody
Anti-Dimethylaniline Oxidase 1 antibody
Anti-Fetal Hepatic Flavin-Containing Monooxygenase 1 antibody
Anti-Fmo 1 antibody
Anti-Trimethylamine Monooxygenase antibody
Anti-FMO1 antibody

Information sourced from Uniprot.org

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