• Immunohistochemistry analysis of paraffin-embedded mouse spleen using FLT1 antibody (STJ111066) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using FLT1 antibody (STJ111066) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using FLT1 antibody (STJ111066) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using VEGFR1 antibody (STJ111066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-FLT1 antibody (20-300) (STJ111066)

SKU:
STJ111066

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VEGF Receptor 1 (20-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLT1
Gene ID: 2321
Uniprot ID: VGFR1_HUMAN
Immunogen Region: 20-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human VEGFR1 (NP_002010.2).
Immunogen Sequence: LTGSSSGSKLKDPELSLKGT QHIMQAGQTLHLQCRGEAAH KWSLPEMVSKESERLSITKS ACGRNGKQFCSTLTLNTAQA NHTGFYSCKYLAVPTSKKKE TESAIYIFISDTGRPFVEMY SEIPEIIHMTEGRELVIPCR VTSPNITVTLKKFPLDTLIP DGKRIIWDSRKGFIISNATY KEIGLLTCEATVNGHLYKTN YLTHRQTNTIIDVQISTPRP VKLLRGHTLVLNCTATTPL
Tissue Specificity Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC).
Post Translational Modifications N-glycosylated. Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-1169 is important for interaction with PLCG. Phosphorylation at Tyr-1213 is important for interaction with PIK3R1, PTPN11, GRB2, and PLCG. Phosphorylation at Tyr-1333 is important for endocytosis and for interaction with CBL, NCK1 and CRK. Is probably dephosphorylated by PTPRB.
Function Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity.may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 1: Phosphorylates PLCG. Isoform 2: May function as decoy receptor for VEGFA. Isoform 3: May function as decoy receptor for VEGFA. Isoform 4: May function as decoy receptor for VEGFA. Isoform 7: Has a truncated kinase domain.it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.
Protein Name Vascular Endothelial Growth Factor Receptor 1
Vegfr-1
Fms-Like Tyrosine Kinase 1
Flt-1
Tyrosine-Protein Kinase Frt
Tyrosine-Protein Kinase Receptor Flt
Flt
Vascular Permeability Factor Receptor
Database Links Reactome: R-HSA-194306
Reactome: R-HSA-195399
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Endosome
Autophosphorylation Promotes Ubiquitination And Endocytosis
Isoform 2: Secreted
Isoform 3: Secreted
Isoform 4: Secreted
Isoform 5: Cytoplasm
Isoform 6: Cytoplasm
Isoform 7: Cytoplasm
Alternative Antibody Names Anti-Vascular Endothelial Growth Factor Receptor 1 antibody
Anti-Vegfr-1 antibody
Anti-Fms-Like Tyrosine Kinase 1 antibody
Anti-Flt-1 antibody
Anti-Tyrosine-Protein Kinase Frt antibody
Anti-Tyrosine-Protein Kinase Receptor Flt antibody
Anti-Flt antibody
Anti-Vascular Permeability Factor Receptor antibody
Anti-FLT1 antibody
Anti-FLT antibody
Anti-FRT antibody
Anti-VEGFR1 antibody

Information sourced from Uniprot.org

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