• Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse pancreas using Flotillin 1 Rabbit polyclonal antibody (STJ11104576) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded rat heart using Flotillin 1 Rabbit polyclonal antibody (STJ11104576) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Flotillin 1 Rabbit polyclonal antibody (STJ11104576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-FLOT1 antibody (128-427) (STJ11104576)

SKU:
STJ11104576

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Flotillin-1 (128-427) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLOT1
Gene ID: 10211
Uniprot ID: FLOT1_HUMAN
Immunogen Region: 128-427
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 128-427 of human Flotillin 1 (NP_005794.1).
Immunogen Sequence: SEQVFKVASSDLVNMGISVV SYTLKDIHDDQDYLHSLGKA RTAQVQKDARIGEAEAKRDA GIREAKAKQEKVSAQYLSEI EMAKAQRDYELKKAAYDIEV NTRRAQADLAYQLQVAKTKQ QIEEQRVQVQVVERAQQVAV QEQEIARREKELEARVRKPA EAERYKLERLAEAEKSQLIM QAEAEAASVRMRGEAEAFAI GARARAEAEQMAKKAEAFQL YQEAAQLDMLLEKLPQVAE
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Protein Name Flotillin-1
Database Links Reactome: R-HSA-5213460
Reactome: R-HSA-5675482
Reactome: R-HSA-8849932
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Endosome
Membrane
Caveola
Melanosome
Membrane Raft
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Membrane-Associated Protein Of Caveola
Alternative Antibody Names Anti-Flotillin-1 antibody
Anti-FLOT1 antibody

Information sourced from Uniprot.org

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