• Immunohistochemistry analysis of FLII in paraffin-embedded mouse heart using FLII Rabbit polyclonal antibody (STJ117468) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FLII in paraffin-embedded human colon carcinoma using FLII Rabbit polyclonal antibody (STJ117468) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FLII in paraffin-embedded rat heart using FLII Rabbit polyclonal antibody (STJ117468) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using FLII Rabbit polyclonal antibody (STJ117468) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5S.

Anti-FLII antibody (400-660) (STJ117468)

SKU:
STJ117468

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLII
Gene ID: 2314
Uniprot ID: FLII_HUMAN
Immunogen Region: 400-660
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human FLII (NP_002009.1).
Immunogen Sequence: LRLAGASPATVAAAAAAGSG PKDPMARKMRLRRRKDSAQD DQAKQVLKGMSDVAQEKNKK QEESADARAPSGKVRRWDQG LEKPRLDYSEFFTEDVGQLP GLTIWQIENFVPVLVEEAFH GKFYEADCYIVLKTFLDDSG SLNWEIYYWIGGEATLDKKA CSAIHAVNLRNYLGAECRTV REEMGDESEEFLQVFDNDIS YIEGGTASGFYTVEDTHYVT RMYRVYGKKNIKLEPVPLK
Tissue Specificity Strongest expression in skeletal muscle with high expression also in the heart and lung.
Function Is a regulator of actin polymerization, required for proper myofibril organization and regulation of the length of sarcomeric thin filaments. It also plays a role in the assembly of cardiomyocyte cell adhesion complexes. Regulates cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation. May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling.
Protein Name Protein Flightless-1 Homolog
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cell Projection
Podosome
Cell Junction
Focal Adhesion
Colocalizes To Actin-Rich Structures In Blastocysts And
Together With Hras
Rhoa And Cdc42
In Migrating Fibroblasts
Localizes To Centrosomes
Localized To The Core Of Macrophage Podosomes
Alternative Antibody Names Anti-Protein Flightless-1 Homolog antibody
Anti-FLII antibody
Anti-FLIL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance