| Short Description : | Rabbit polyclonal FLII (400-660) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:200-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FLII |
| Gene ID: | 2314 |
| Uniprot ID: | FLII_HUMAN |
| Immunogen Region: | 400-660 |
| Immunogen Sequence: | LRLAGASPATVAAAAAAGSG PKDPMARKMRLRRRKDSAQD DQAKQVLKGMSDVAQEKNKK QEESADARAPSGKVRRWDQG LEKPRLDYSEFFTEDVGQLP GLTIWQIENFVPVLVEEAFH GKFYEADCYIVLKTFLDDSG SLNWEIYYWIGGEATLDKKA CSAIHAVNLRNYLGAECRTV REEMGDESEEFLQVFDNDIS YIEGGTASGFYTVEDTHYVT RMYRVYGKKNIKLEPVPLK |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human FLII (NP_002009.1). |
| Tissue Specificity | Strongest expression in skeletal muscle with high expression also in the heart and lung. |
| Function | Is a regulator of actin polymerization, required for proper myofibril organization and regulation of the length of sarcomeric thin filaments. It also plays a role in the assembly of cardiomyocyte cell adhesion complexes. Regulates cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation. May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. |
| Protein Name | Protein Flightless-1 Homolog |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCell ProjectionPodosomeCell JunctionFocal AdhesionColocalizes To Actin-Rich Structures In Blastocysts AndTogether With HrasRhoa And Cdc42In Migrating FibroblastsLocalizes To CentrosomesLocalized To The Core Of Macrophage Podosomes |
| Alternative Antibody Names | Anti-Protein Flightless-1 Homolog antibodyAnti-FLII antibodyAnti-FLIL antibody |
Information sourced from Uniprot.org
