• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-FLG antibody (STJ192986)

SKU:
STJ192986

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Filaggrin is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-300
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLG
Gene ID: 2312
Uniprot ID: FILA_HUMAN
Specificity: FILA Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from part of the human protein
Post Translational Modifications Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate. Undergoes deimination of some arginine residues (citrullination).
Function Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
Protein Name Filaggrin
Database Links Reactome: R-HSA-6809371
Cellular Localisation Cytoplasmic Granule
In The Stratum Granulosum Of The Epidermis
Localized Within Keratohyalin Granules
In Granular Keratinocytes And In Lower Corneocytes
Colocalizes With Calpain-1/Capn1
Alternative Antibody Names Anti-Filaggrin antibody
Anti-FLG antibody

Information sourced from Uniprot.org

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