| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FLCN (341-579) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FLCN |
| Gene ID: |
201163 |
| Uniprot ID: |
FLCN_HUMAN |
| Immunogen Region: |
341-579 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 341-579 of human FLCN (NP_659434.2). |
| Immunogen Sequence: |
RKLPVFKSLRHMRQVLGAPS FRMLAWHVLMGNQVIWKSRD VDLVQSAFEVLRTMLPVGCV RIIPYSSQYEEAYRCNFLGL SPHVQIPPHVLSSEFAVIVE VHAAARSTLHPVGCEDDQSL SKYEFVVTSGSPVAADRVGP TILNKIEAALTNQNLSVDVV DQCLVCLKEEWMNKVKVLFK FTKVDSRPKEDTQKLLSILG ASEEDNVKLLKFWMTGLSKT YKSHLMSTVRSPTASESRN |
| Tissue Specificity | Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach. |
| Post Translational Modifications | Phosphorylation by ULK1 modulates the interaction with GABARAP and is required to regulate autophagy. |
| Function | Multi-functional protein, involved in both the cellular response to amino acid availability and in the regulation of glycolysis. GTPase-activating protein that plays a key role in the cellular response to amino acid availability through regulation of the non-canonical mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3. Activates mTORC1 by acting as a GTPase-activating protein: specifically stimulates GTP hydrolysis by RagC/RRAGC or RagD/RRAGD, promoting the conversion to the GDP-bound state of RagC/RRAGC or RagD/RRAGD, and thereby activating the kinase activity of mTORC1. The GTPase-activating activity is inhibited during starvation and activated in presence of nutrients. Acts as a key component for non-canonical mTORC1-dependent control of the MiT/TFE factors TFEB and TFE3, while it is not involved in mTORC1-dependent phosphorylation of canonical RPS6KB1/S6K1 and EIF4EBP1/4E-BP1. In low-amino acid conditions, the lysosomal folliculin complex (LFC) is formed on the membrane of lysosomes, which inhibits the GTPase-activating activity of FLCN, inactivates mTORC1 and maximizes nuclear translocation of TFEB and TFE3. Upon amino acid restimulation, RagA/RRAGA (or RagB/RRAGB) nucleotide exchange promotes disassembly of the LFC complex and liberates the GTPase-activating activity of FLCN, leading to activation of mTORC1 and subsequent cytoplasmic retention of TFEB and TFE3. Indirectly acts as a positive regulator of Wnt signaling by promoting mTOR-dependent cytoplasmic retention of MiT/TFE factor TFE3. Required for the exit of hematopoietic stem cell from pluripotency by promoting mTOR-dependent cytoplasmic retention of TFE3, thereby increasing Wnt signaling. Acts as an inhibitor of browning of adipose tissue by regulating mTOR-dependent cytoplasmic retention of TFE3. Involved in the control of embryonic stem cells differentiation.together with LAMTOR1 it is necessary to recruit and activate RagC/RRAGC and RagD/RRAGD at the lysosomes, and to induce exit of embryonic stem cells from pluripotency via non-canonical, mTOR-independent TFE3 inactivation. In response to flow stress, regulates STK11/LKB1 accumulation and mTORC1 activation through primary cilia: may act by recruiting STK11/LKB1 to primary cilia for activation of AMPK resided at basal bodies, causing mTORC1 down-regulation. Together with FNIP1 and/or FNIP2, regulates autophagy: following phosphorylation by ULK1, interacts with GABARAP and promotes autophagy. Required for starvation-induced perinuclear clustering of lysosomes by promoting association of RILP with its effector RAB34. Regulates glycolysis by binding to lactate dehydrogenase LDHA, acting as an uncompetitive inhibitor. |
| Protein Name | FolliculinBhd Skin Lesion Fibrofolliculoma ProteinBirt-Hogg-Dube Syndrome Protein |
| Database Links | Reactome: R-HSA-9639288 |
| Cellular Localisation | Lysosome MembraneCytoplasmCytosolCell ProjectionCiliumCytoskeletonMicrotubule Organizing CenterCentrosomeSpindleNucleusLocalizes To Lysosome Membrane In Amino Acid-Depleted Conditions And Relocalizes To The Cytosol Upon RefeedingColocalizes With Fnip1 And Fnip2 In The CytoplasmAlso Localizes To Motile And Non-Motile CiliaCentrosomes And The Mitotic Spindle |
| Alternative Antibody Names | Anti-Folliculin antibodyAnti-Bhd Skin Lesion Fibrofolliculoma Protein antibodyAnti-Birt-Hogg-Dube Syndrome Protein antibodyAnti-FLCN antibodyAnti-BHD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
