| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SEPN1 (341-590) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SELENON |
| Gene ID: |
57190 |
| Uniprot ID: |
SELN_HUMAN |
| Immunogen Region: |
341-590 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 341-590 of human SEPN1 (NP_065184.2). |
| Immunogen Sequence: |
VDMEWLYGASESSNMEVDIG YIPQMELEATGPSVPSVILD EDGSMIDSHLPSGEPLQFVF EEIKWQQELSWEEAARRLEV AMYPFKKVSYLPFTEAFDRA KAENKLVHSILLWGALDDQS CUGSGRTLRETVLESSPILT LLNESFISTWSLVKELEELQ NNQENSSHQKLAGLHLEKYS FPVEMMICLPNGTVVHHINA NYFLDITSVKPEEIESNLFS FSSTFEDPSTATYMQFLKE |
| Tissue Specificity | Isoform 1 and isoform 2 are expressed in skeletal muscle, brain, lung and placenta. Isoform 2 is also expressed in heart, diaphragm and stomach. |
| Post Translational Modifications | Isoform 2: N-glycosylated. |
| Function | Isoform 2: Plays an important role in cell protection against oxidative stress and in the regulation of redox-related calcium homeostasis. Regulates the calcium level of the ER by protecting the calcium pump ATP2A2 against the oxidoreductase ERO1A-mediated oxidative damage. Within the ER, ERO1A activity increases the concentration of H(2)O(2), which attacks the luminal thiols in ATP2A2 and thus leads to cysteinyl sulfenic acid formation (-SOH) and SEPN1 reduces the SOH back to free thiol (-SH), thus restoring ATP2A2 activity. Acts as a modulator of ryanodine receptor (RyR) activity: protects RyR from oxidation due to increased oxidative stress, or directly controls the RyR redox state, regulating the RyR-mediated calcium mobilization required for normal muscle development and differentiation. Essential for muscle regeneration and satellite cell maintenance in skeletal muscle. |
| Protein Name | Selenoprotein NSeln |
| Cellular Localisation | Isoform 2: Endoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-Selenoprotein N antibodyAnti-Seln antibodyAnti-SELENON antibodyAnti-SELN antibodyAnti-SEPN1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
