• Immunofluorescence analysis of A-549 cells using FIP1L1 antibody (STJ29218).
  • Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using FIP1L1 antibody (STJ29218) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FIP1L1 antibody (STJ29218) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat testis using FIP1L1 antibody (STJ29218) at dilution of 1:100 (20x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using FIP1L1 Rabbit polyclonal antibody (STJ29218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FIP1L1 antibody (1-300) (STJ29218)

SKU:
STJ29218

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FIP1L1 (1-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FIP1L1
Gene ID: 81608
Uniprot ID: FIP1_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human FIP1L1 (NP_001128409.1).
Immunogen Sequence: MSAGEVERLVSELSGGTGGD EEEEWLYGDENEVERPEEEN ASANPPSGIEDETAENGVPK PKVTETEDDSDSDSDDDEDD VHVTIGDIKTGAPQYGSYGT APVNLNIKTGGRVYGTTGTK VKGVDLDAPGSINGVPLLEV DLDSFEDKPWRKPGADLSDY FNYGFNEDTWKAYCEKQKRI RMGLEVIPVTSTTNKITAED CTMEVTPGAEIQDGRFNLFK VQQGRTGNSEKETALPSTK
Function Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 contributes to poly(A) site recognition and stimulates poly(A) addition. Binds to U-rich RNA sequence elements surrounding the poly(A) site. May act to tether poly(A) polymerase to the CPSF complex.
Protein Name Pre-Mrna 3'-End-Processing Factor Fip1
Hfip1
Fip1-Like 1 Protein
Factor Interacting With Pap
Rearranged In Hypereosinophilia
Database Links Reactome: R-HSA-159231
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77595
Reactome: R-HSA-9673766
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pre-Mrna 3'-End-Processing Factor Fip1 antibody
Anti-Hfip1 antibody
Anti-Fip1-Like 1 Protein antibody
Anti-Factor Interacting With Pap antibody
Anti-Rearranged In Hypereosinophilia antibody
Anti-FIP1L1 antibody
Anti-FIP1 antibody
Anti-RHE antibody

Information sourced from Uniprot.org

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