• Western blot analysis of lysates from wild type (WT) and 293T cells transfected with FGF23 using FGF23 Rabbit polyclonal antibody (STJ116008) at 1:600 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 20  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 1s.

Anti-FGF23 antibody (91-190) (STJ116008)

SKU:
STJ116008

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FGF23 (91-190) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FGF23
Gene ID: 8074
Uniprot ID: FGF23_HUMAN
Immunogen Region: 91-190
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 91-190 of human FGF23 (NP_065689.1).
Immunogen Sequence: RRYLCMDFRGNIFGSHYFDP ENCRFQHQTLENGYDVYHSP QYHFLVSLGRAKRAFLPGMN PPPYSQFLSRRNEIPLIHFN TPIPRRHTRSAEDDSERDPL
Tissue Specificity Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).
Post Translational Modifications Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases. O-glycosylated at Thr-171 and Thr-178 by GALNT3 and glycosylation of Thr-178 requires previous glycosylation at Thr171. Glycosylation is necessary for secretion.it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. Phosphorylation at Ser-180 mediated by FAM20C slows down glycosylation at Thr-178 notably.
Function Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Up-regulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.
Protein Name Fibroblast Growth Factor 23
Fgf-23
Phosphatonin
Tumor-Derived Hypophosphatemia-Inducing Factor Cleaved Into - Fibroblast Growth Factor 23 N-Terminal Peptide - Fibroblast Growth Factor 23 C-Terminal Peptide
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-1839122
Reactome: R-HSA-1839130
Reactome: R-HSA-190322
Reactome: R-HSA-190372
Reactome: R-HSA-190373
Reactome: R-HSA-190374
Reactome: R-HSA-190375
Reactome: R-HSA-2033519
Reactome: R-HSA-2219530
Reactome: R-HSA-381426
Reactome: R-HSA-5654219
Reactome: R-HSA-5654221
Reactome: R-HSA-5654227
Reactome: R-HSA-5654228
Reactome: R-HSA-5654687
Reactome: R-HSA-5654688
Reactome: R-HSA-5654689
Reactome: R-HSA-5654693
Reactome: R-HSA-5654695
Reactome: R-HSA-5654699
Reactome: R-HSA-5654700
Reactome: R-HSA-5654704
Reactome: R-HSA-5654706
Reactome: R-HSA-5654710
Reactome: R-HSA-5654712
Reactome: R-HSA-5654719
Reactome: R-HSA-5654720
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-5655253
Reactome: R-HSA-5655302
Reactome: R-HSA-5655332
Reactome: R-HSA-5658623
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Secretion Is Dependent On O-Glycosylation
Alternative Antibody Names Anti-Fibroblast Growth Factor 23 antibody
Anti-Fgf-23 antibody
Anti-Phosphatonin antibody
Anti-Tumor-Derived Hypophosphatemia-Inducing Factor Cleaved Into - Fibroblast Growth Factor 23 N-Terminal Peptide - Fibroblast Growth Factor 23 C-Terminal Peptide antibody
Anti-FGF23 antibody
Anti-HYPF antibody
Anti-UNQ3027 antibody
Anti-PRO9828 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance