• Western blot analysis of extracts of various cell lines, using FECH antibody (STJ110716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using FECH antibody (STJ110716) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human stomach using FECH antibody (STJ110716) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using FECH antibody (STJ110716) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using FECH antibody (STJ110716) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat heart using FECH antibody (STJ110716) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse lung using FECH antibody (STJ110716) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human stomach using FECH antibody (STJ110716) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse stomach using FECH antibody (STJ110716) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using FECH antibody (STJ110716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-FECH antibody (1-200) (STJ110716)

SKU:
STJ110716

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FECH (1-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FECH
Gene ID: 2235
Uniprot ID: HEMH_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human FECH (NP_000131.2).
Immunogen Sequence: MRSLGANMAAALRAAGVLLR DPLASSSWRVCQPWRWKSGA AAAAVTTETAQHAQGAKPQV QPQKRKPKTGILMLNMGGPE TLGDVHDFLLRLFLDRDLMT LPIQNKLAPFIAKRRTPKIQ EQYRRIGGGSPIKIWTSKQG EGMVKLLDELSPNTAPHKYY IGFRYVHPLTEEAIEEMERD GLERAIAFTQYPQYSCSTTG
Function Catalyzes the ferrous insertion into protoporphyrin IX.
Protein Name Ferrochelatase - Mitochondrial
Heme Synthase
Protoheme Ferro-Lyase
Database Links Reactome: R-HSA-189451
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Ferrochelatase - Mitochondrial antibody
Anti-Heme Synthase antibody
Anti-Protoheme Ferro-Lyase antibody
Anti-FECH antibody

Information sourced from Uniprot.org

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