• Immunofluorescence analysis of paraffin-embedded Human adrenal cortex tissue using Ferredoxin Reductase Rabbit polyclonal antibody (STJ11105628) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using Ferredoxin Reductase Rabbit polyclonal antibody (STJ11105628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of lysates from Hep G2 cells using Ferredoxin Reductase Rabbit polyclonal antibody (STJ11105628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FDXR antibody (142-491) (STJ11105628)

SKU:
STJ11105628

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nadph-Adrenodoxin Oxidoreductase-Mitochondrial (142-491) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FDXR
Gene ID: 2232
Uniprot ID: ADRO_HUMAN
Immunogen Region: 142-491
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 142-491 of human Ferredoxin Reductase (NP_077728.3).
Immunogen Sequence: EIPGEELPGVCSARAFVGWY NGLPENQELEPDLSCDTAVI LGQGNVALDVARILLTPPEH LERTDITKAALGVLRQSRVK TVWLVGRRGPLQVAFTIKEL REMIQLPGARPILDPVDFLG LQDKIKEVPRPRKRLTELLL RTATEKPGPAEAARQASASR AWGLRFFRSPQQVLPSPDGR RAAGVRLAVTRLEGVDEATR AVPTGDMEDLPCGLVLSSIG YKSRPVDPSVPFDSKLGVI
Function Serves as the first electron transfer protein in all the mitochondrial P450 systems including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.
Protein Name Nadph -Adrenodoxin Oxidoreductase - Mitochondrial
Ar
Adrenodoxin Reductase
Ferredoxin--Nadp(+ Reductase
Ferredoxin Reductase
Database Links Reactome: R-HSA-196108
Reactome: R-HSA-211976
Reactome: R-HSA-2395516
Reactome: R-HSA-5579026
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Nadph -Adrenodoxin Oxidoreductase - Mitochondrial antibody
Anti-Ar antibody
Anti-Adrenodoxin Reductase antibody
Anti-Ferredoxin--Nadp(+ Reductase antibody
Anti-Ferredoxin Reductase antibody
Anti-FDXR antibody
Anti-ADXR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance