• Western blot analysis of extracts of various cell lines, using FDX1/ADX antibody (STJ111857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using FDX1/ADX antibody (STJ111857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-FDX1 antibody (1-184) (STJ111857)

SKU:
STJ111857

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FDX1 (1-184) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FDX1
Gene ID: 2230
Uniprot ID: ADX_HUMAN
Immunogen Region: 1-184
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human FDX1/ADX (NP_004100.1).
Immunogen Sequence: MAAAGGARLLRAASAVLGGP AGRWLHHAGSRAGSSGLLRN RGPGGSAEASRSLSVSARAR SSSEDKITVHFINRDGETLT TKGKVGDSLLDVVVENNLDI DGFGACEGTLACSTCHLIFE DHIYEKLDAITDEENDMLDL AYGLTDRSRLGCQICLTKSM DNMTVRVPETVADARQSIDV GKTS
Tissue Specificity Highest levels in the adrenal gland (at protein level). Also detected in kidney and testis (at protein level).
Function Essential for the synthesis of various steroid hormones. Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage. Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons.
Protein Name Adrenodoxin - Mitochondrial
Adrenal Ferredoxin
Ferredoxin-1
Hepatoredoxin
Database Links Reactome: R-HSA-1362409
Reactome: R-HSA-196108
Reactome: R-HSA-211976
Reactome: R-HSA-2395516
Reactome: R-HSA-5579026
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Adrenodoxin - Mitochondrial antibody
Anti-Adrenal Ferredoxin antibody
Anti-Ferredoxin-1 antibody
Anti-Hepatoredoxin antibody
Anti-FDX1 antibody
Anti-ADX antibody

Information sourced from Uniprot.org

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