• Western blot analysis of extracts of various cell lines, using FBXO6 antibody (STJ118010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunofluorescence analysis of NIH/3T3 cells using FBXO6 rabbit polyclonal antibody (STJ118010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-FBXO6 antibody (70-260) (STJ118010)

SKU:
STJ118010

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FBXO6 (70-260) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FBXO6
Gene ID: 26270
Uniprot ID: FBX6_HUMAN
Immunogen Region: 70-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 70-260 of human FBXO6 (NP_060908.1).
Immunogen Sequence: QPVADWKIFYFLRSLHRNLL RNPCAEEDMFAWQIDFNGGD RWKVESLPGAHGTDFPDPKV KKYFVTSYEMCLKSQLVDLV AEGYWEELLDTFRPDIVVKD WFAARADCGCTYQLKVQLAS ADYFVLASFEPPPVTIQQWN NATWTEVSYTFSDYPRGVRY ILFQHGGRDTQYWAGWYGPR VTNSSIVVSPK
Function Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex-type oligosaccharides. Also recognizes sulfated glycans. Also involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to ensure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication.
Protein Name F-Box Only Protein 6
F-Box Protein That Recognizes Sugar Chains 2
F-Box/G-Domain Protein 2
Database Links Reactome: R-HSA-390471
Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-F-Box Only Protein 6 antibody
Anti-F-Box Protein That Recognizes Sugar Chains 2 antibody
Anti-F-Box/G-Domain Protein 2 antibody
Anti-FBXO6 antibody
Anti-FBG2 antibody
Anti-FBS2 antibody
Anti-FBX6 antibody

Information sourced from Uniprot.org

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