| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FBXO3 |
| Gene ID: | 26273 |
| Uniprot ID: | FBX3_HUMAN |
| Immunogen Region: | 1-240 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human FBXO3 (NP_036307.2). |
| Immunogen Sequence: | MAAMETETAPLTLESLPTDP LLLILSFLDYRDLINCCYVS RRLSQLSSHDPLWRRHCKKY WLISEEEKTQKNQCWKSLFI DTYSDVGRYIDHYAAIKKAW DDLKKYLEPRCPRMVLSLKE GAREEDLDAVEAQIGCKLPD DYRCSYRIHNGQKLVVPGLL GSMALSNHYRSEDLLDVDTA AGGFQQRQGLKYCLPLTFCI HTGLSQYIAVEAAEGRNKNE VFYQCPDQMARNPAAIDMF |
| Function | Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, SCF(FBXO3), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. The SCF(FBXO3) also acts as a regulator of inflammation by mediating ubiquitination and degradation of FBXL2 in response to lipopolysaccharide (LPS). The SCF(FBXO3) complex specifically recognizes FBXL2 phosphorylated at 'Thr-404' and promotes its ubiquitination. |
| Protein Name | F-Box Only Protein 3 |
| Cellular Localisation | Nucleus Colocalizes With Pml At The Peripheries Of Nuclear Bodies |
| Alternative Antibody Names | Anti-F-Box Only Protein 3 antibody Anti-FBXO3 antibody Anti-FBX3 antibody |
Information sourced from Uniprot.org