| Host: | Rabbit |
| Applications: | ELISA/IHC/IP/WB |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Fbx32 (240-280) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.64-0.72 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:50-1:150 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Arf1 |
| Gene ID: | 64310 |
| Uniprot ID: | ARF1_RAT |
| Immunogen Region: | 240-280 |
| Immunogen: | Synthetic peptide taken within amino acid region 240-280 on Atr1 protein. |
| Function | Small GTPase involved in protein trafficking between different compartments. Modulates vesicle budding and uncoating within the Golgi complex. In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity.the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis. Plays also a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation. |
| Protein Name | Adp-Ribosylation Factor 1 |
| Database Links | Reactome: R-RNO-1660499 Reactome: -RNO-1660514 Reactome: -RNO-199992 Reactome: -RNO-2132295 Reactome: -RNO-432720 Reactome: -RNO-432722 Reactome: -RNO-6807878 Reactome: -RNO-6811434 Reactome: -RNO-6811438 Reactome: -RNO-9845576 |
| Cellular Localisation | Golgi Apparatus Membrane Lipid-Anchor Cytoplasmic Side Synapse Synaptosome Postsynaptic Density In The Gdp-Bound Form Associates Transiently With The Membranes Via Its Myristoylated N-Terminus Where Guanine Nucleotide-Exchange Factor (Gef)-Mediated Nucleotide Exchange Occurs Following Nucleotide Exchange The Gtp-Bound Form Undergoes A Conformational Change Leading To The Exposure Of A Myristoylated N-Terminal Amphipathic Helix That Provides Stable Membrane Anchorage |
| Alternative Antibody Names | Anti-Adp-Ribosylation Factor 1 antibody Anti-Arf1 antibody |
Information sourced from Uniprot.org