• STJ70401 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70401 (8µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ70401 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

Anti-FBL2/FBXL2 antibody (N-Term) (STJ70401)

SKU:
STJ70401-100

£312.50
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Host: Goat
Applications: Pep-ELISA/IHC/IF
Reactivity: Human/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-FBL2/FBXL2 (N-Term) is suitable for use in ELISA, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Preliminary testing showed a band at approx 48kDa in Human Kidney lysate after 0.1µg/ml antibody staining (calculated MW of 47.1kDa according to NP_036289.2). Primary incubation 1 hou
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: FBXL2
Gene ID: 25827
Uniprot ID: FBXL2_HUMAN
Immunogen Region: N-Term
Accession Number: NP_036289.2
Immunogen Sequence: VFSNNDEGLINKK
Post Translational Modifications Phosphorylated by GSK-beta (GSK3B), promoting recognition by FBXO3, leading to its ubiquitination by the SCF(FBXO3) complex. Ubiquitinated at Lys-201 by the SCF(FBXO3) complex in response to lipopolysaccharide (LPS), leading to its degradation by the proteasome.
Function Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy. PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant. The SCF(FBXL2) complex acts as a regulator of inflammation by mediating ubiquitination and degradation of TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF4, TRAF5 and TRAF6). The SCF(FBXL2) complex acts as a negative regulator of the NLRP3 inflammasome by mediating ubiquitination and degradation of NLRP3.
Protein Name F-Box/Lrr-Repeat Protein 2
F-Box And Leucine-Rich Repeat Protein 2
F-Box Protein Fbl2/Fbl3
Cellular Localisation Membrane
Lipid-Anchor
Alternative Antibody Names Anti-F-Box/Lrr-Repeat Protein 2 antibody
Anti-F-Box And Leucine-Rich Repeat Protein 2 antibody
Anti-F-Box Protein Fbl2/Fbl3 antibody
Anti-FBXL2 antibody
Anti-FBL2 antibody
Anti-FBL3 antibody

Information sourced from Uniprot.org

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