• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Fatty Acid Synthase (FASN) antibody (STJ11101588). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase (FASN) antibody (STJ11101588) at a dilution of 1:500.
  • Immunofluorescence analysis of HeLa cells using Fatty Acid Synthase (FASN) Rabbit monoclonal antibody (STJ11101588) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Fatty Acid Synthase (FASN) Rabbit monoclonal antibody (STJ11101588) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Fatty Acid Synthase (FASN) in paraffin-embedded human colon carcinoma using Fatty Acid Synthase (FASN) Rabbit monoclonal antibody (STJ11101588) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Fatty Acid Synthase (FASN) Rabbit monoclonal antibody (STJ11101588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-FASN antibody (2400-2500) [S8MR] (STJ11101588)

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STJ11101588

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FASN (2400-2500) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FASN
Gene ID: 2194
Uniprot ID: FAS_HUMAN
Immunogen Region: 2400-2500
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2400-2500 of human FASN (P49327).
Immunogen Sequence: AVDLIIKSHQGLDRQELSFA ARSFYYKLRAAEQYTPKAKY HGNVMLLRAKTGGAYGEDLG ADYNLSQVCDGKVSVHVIEG DHRTLLEGSGLESIISIIHS S
Tissue Specificity Ubiquitous. Prominent expression in brain, lung, liver and mammary gland.
Post Translational Modifications S-nitrosylation of Fatty acid synthase at cysteine residues Cys-1471 or Cys-2091 is important for the enzyme dimerization. In adipocytes, S-nitrosylation of Fatty acid synthase occurs under physiological conditions and gradually increases during adipogenesis.
Function Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein (ACP) domain. (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
Protein Name Fatty Acid Synthase
Type I Fatty Acid Synthase Includes - Acyl-Carrier-Protein S-Acetyltransferase - Acyl-Carrier-Protein S-Malonyltransferase - 3-Oxoacyl-Acyl-Carrier-Protein Synthase - 3-Oxoacyl-Acyl-Carrier-Protein Reductase - 3-Hydroxyacyl-Acyl-Carrier-Protein Dehydratase - Enoyl-Acyl-Carrier-Protein Reductase - Acyl-Acyl-Carrier-Protein Hydrolase
Database Links Reactome: R-HSA-163765
Reactome: R-HSA-199220
Reactome: R-HSA-2426168
Reactome: R-HSA-75105
Reactome: R-HSA-9029558
Cellular Localisation Cytoplasm
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Fatty Acid Synthase antibody
Anti-Type I Fatty Acid Synthase Includes - Acyl-Carrier-Protein S-Acetyltransferase - Acyl-Carrier-Protein S-Malonyltransferase - 3-Oxoacyl-Acyl-Carrier-Protein Synthase - 3-Oxoacyl-Acyl-Carrier-Protein Reductase - 3-Hydroxyacyl-Acyl-Carrier-Protein Dehydratase - Enoyl-Acyl-Carrier-Protein Reductase - Acyl-Acyl-Carrier-Protein Hydrolase antibody
Anti-FASN antibody
Anti-FAS antibody

Information sourced from Uniprot.org

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