• Immunofluorescence analysis of PC-12 cells using FAF2 Rabbit polyclonal antibody (STJ11103328) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using FAF2 antibody (STJ11103328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FAF2 antibody (176-393) (STJ11103328)

SKU:
STJ11103328

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FAF2 (176-393) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FAF2
Gene ID: 23197
Uniprot ID: FAF2_HUMAN
Immunogen Region: 176-393
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 176-393 of human FAF2 (NP_055428.1).
Immunogen Sequence: YLHGDDHQDSDEFCRNTLCA PEVISLINTRMLFWACSTNK PEGYRVSQALRENTYPFLAM IMLKDRRMTVVGRLEGLIQP DDLINQLTFIMDANQTYLVS ERLEREERNQTQVLRQQQDE AYLASLRADQEKERKKREER ERKRRKEEEVQQQKLAEERR RQNLQEEKERKLECLPPEPS PDDPESVKIIFKLPNDSRVE RRFHFSQSLTVIHDFLFS
Tissue Specificity Broadly expressed, with highest levels in brain.
Function Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway. Involved in inhibition of lipid droplet degradation by binding to phospholipase PNPL2 and inhibiting its activity by promoting dissociation of PNPL2 from its endogenous activator, ABHD5 which inhibits the rate of triacylglycerol hydrolysis. Involved in stress granule disassembly: associates with ubiquitinated G3BP1 in response to heat shock, thereby promoting interaction between ubiquitinated G3BP1 and VCP, followed by G3BP1 extraction from stress granules and stress granule disassembly.
Protein Name Fas-Associated Factor 2
Ubx Domain-Containing Protein 3b
Ubx Domain-Containing Protein 8
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8980692
Cellular Localisation Cytoplasm
Lipid Droplet
Endoplasmic Reticulum
Alternative Antibody Names Anti-Fas-Associated Factor 2 antibody
Anti-Ubx Domain-Containing Protein 3b antibody
Anti-Ubx Domain-Containing Protein 8 antibody
Anti-FAF2 antibody
Anti-ETEA antibody
Anti-KIAA0887 antibody
Anti-UBXD8 antibody
Anti-UBXN3B antibody

Information sourced from Uniprot.org

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