• Western blot analysis of extracts of various cell lines, using FADD rabbit monoclonal antibody (STJ11103172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using FADD rabbit monoclonal antibody (STJ11103172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using FADD rabbit monoclonal antibody (STJ11103172) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung using FADD rabbit monoclonal antibody (STJ11103172) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using FADD rabbit monoclonal antibody (STJ11103172) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using FADD rabbit monoclonal antibody (STJ11103172) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-FADD antibody (1-205) [S2MR] (STJ11103172)

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STJ11103172

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FADD (1-205) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Fadd
Gene ID: 14082
Uniprot ID: FADD_MOUSE
Immunogen Region: 1-205
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of mouse FADD (NP_034305.1).
Immunogen Sequence: MDPFLVLLHSLSGSLSGNDL MELKFLCRERVSKRKLERVQ SGLDLFTVLLEQNDLERGHT GLLRELLASLRRHDLLQRLD DFEAGTATAAPPGEADLQVA FDIVCDNVGRDWKRLARELK VSEAKMDGIEEKYPRSLSER VRESLKVWKNAEKKNASVAG LVKALRTCRLNLVADLVEEA QESVSKSENMSPVLRDSTVS SSETP
Function Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
Protein Name Fas-Associated Death Domain Protein
Fas-Associating Death Domain-Containing Protein
Mediator Of Receptor Induced Toxicity
Database Links Reactome: R-MMU-140534
Reactome: -MMU-2562578
Reactome: -MMU-3371378
Reactome: -MMU-5218900
Reactome: -MMU-5357786
Reactome: -MMU-5357905
Reactome: -MMU-5675482
Reactome: -MMU-69416
Reactome: -MMU-75157
Reactome: -MMU-75158
Alternative Antibody Names Anti-Fas-Associated Death Domain Protein antibody
Anti-Fas-Associating Death Domain-Containing Protein antibody
Anti-Mediator Of Receptor Induced Toxicity antibody
Anti-Fadd antibody
Anti-Mort1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance