• Western blot analysis of extracts of BxPC-3 cells, using Coagulation factor III/Tissue Factor antibody (STJ23598) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using Coagulation factor III/Tissue Factor antibody (STJ23598) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of A431 cells using Coagulation factor III/Tissue Factor rabbit polyclonal antibody (STJ23598) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-F3 antibody (120-251) (STJ23598)

SKU:
STJ23598

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-F3 (120-251) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: F3
Gene ID: 2152
Uniprot ID: TF_HUMAN
Immunogen Region: 120-251
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-251 of human Coagulation factor III/Tissue Factor (NP_001984.1).
Immunogen Sequence: SAGEPLYENSPEFTPYLETN LGQPTIQSFEQVGTKVNVTV EDERTLVRRNNTFLSLRDVF GKDLIYTLYYWKSSSSGKKT AKTNTNEFLIDVDKGENYCF SVQAVIPSRTVNRKSTDSPV ECMGQEKGEFRE
Tissue Specificity Lung, placenta and pancreas.
Function Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The TF:VIIa complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Protein Name Tissue Factor
Tf
Coagulation Factor Iii
Thromboplastin
Cd Antigen Cd142
Database Links Reactome: R-HSA-140834
Reactome: R-HSA-9031628
Cellular Localisation Isoform 1: Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative Antibody Names Anti-Tissue Factor antibody
Anti-Tf antibody
Anti-Coagulation Factor Iii antibody
Anti-Thromboplastin antibody
Anti-Cd Antigen Cd142 antibody
Anti-F3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance