| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-F2R/PAR1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 48kDa band observed in lysates of cell line HeLa (calculated MW of 47.4kDa according to NP_001983.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room te |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
F2R |
| Gene ID: |
2149 |
| Uniprot ID: |
PAR1_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_001983.2 |
| Immunogen Sequence: |
TRARRPESKATNAT |
| Post Translational Modifications | Proteolytic cleavage by thrombin generates a new N-terminus that functions as a tethered ligand. Also proteolytically cleaved by cathepsin CTSG. Cleavage at 41-Arg-|-Ser-42 by CTSG results in receptor activation while cleavage at 55-Phe-|-Trp-56 results in inhibition of receptor activation. Phosphorylated in the C-terminal tail.probably mediating desensitization prior to the uncoupling and internalization of the receptor. |
| Function | High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores. The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores. In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation. May play a role in platelets activation and in vascular development. Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells. |
| Protein Name | Proteinase-Activated Receptor 1Par-1Coagulation Factor Ii ReceptorThrombin Receptor |
| Database Links | Reactome: R-HSA-140875Reactome: R-HSA-375276Reactome: R-HSA-416476Reactome: R-HSA-456926 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Proteinase-Activated Receptor 1 antibodyAnti-Par-1 antibodyAnti-Coagulation Factor Ii Receptor antibodyAnti-Thrombin Receptor antibodyAnti-F2R antibodyAnti-CF2R antibodyAnti-PAR1 antibodyAnti-TR antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
