Anti-ETHE1 antibody (8-245) (STJ112181)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ETHE1 (8-245) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ETHE1 |
| Gene ID | 23474 |
| Uniprot ID | ETHE1_HUMAN |
| Immunogen Region | 8-245 |
| Immunogen Sequence | VARRQLSQRGGSGAPILLRQ MFEPVSCTFTYLLGDRESRE AVLIDPVLETAPRDAQLIKE LGLRLLYAVNTHCHADHITG SGLLRSLLPGCQSVISRLSG AQADLHIEDGDSIRFGRFAL ETRASPGHTPGCVTFVLNDH SMAFTGDALLIRGCGRTDFQ QGCAKTLYHSVHEKIFTLPG DCLIYPAHDYHGFTVSTVEE ERTLNPRLTLSCEEFVKIMG NLNLPKPQQIDFAVPANM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 8-245 of human ETHE1 (NP_055112.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Function | Sulfur dioxygenase that plays an essential role in hydrogen sulfide catabolism in the mitochondrial matrix. Hydrogen sulfide (H(2)S) is first oxidized by SQRDL, giving rise to cysteine persulfide residues. ETHE1 consumes molecular oxygen to catalyze the oxidation of the persulfide, once it has been transferred to a thiophilic acceptor, such as glutathione (R-SSH). Plays an important role in metabolic homeostasis in mitochondria by metabolizing hydrogen sulfide and preventing the accumulation of supraphysiological H(2)S levels that have toxic effects, due to the inhibition of cytochrome c oxidase. First described as a protein that can shuttle between the nucleus and the cytoplasm and suppress p53-induced apoptosis by sequestering the transcription factor RELA/NFKB3 in the cytoplasm and preventing its accumulation in the nucleus. |
| Protein Name | Persulfide Dioxygenase Ethe1 - MitochondrialEthylmalonic Encephalopathy Protein 1Hepatoma Subtracted Clone One ProteinSulfur Dioxygenase Ethe1 |
| Database Links | Reactome: R-HSA-1614517 |
| Cellular Localisation | CytoplasmNucleusMitochondrion Matrix |
| Alternative Antibody Names | Anti-Persulfide Dioxygenase Ethe1 - Mitochondrial antibodyAnti-Ethylmalonic Encephalopathy Protein 1 antibodyAnti-Hepatoma Subtracted Clone One Protein antibodyAnti-Sulfur Dioxygenase Ethe1 antibodyAnti-ETHE1 antibodyAnti-HSCO antibody |
Information sourced from Uniprot.org