Anti-ERK3/MAPK6 antibody (Internal) (STJ72939)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ERK3/MAPK6 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation research applications. |
| Applications | Pep-ELISA/WB-Trf/IHC/IP |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: In transfected HEK293 transiently expressing Mouse ERK3/Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands a |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | MAPK6 |
| Gene ID | 5597 |
| Uniprot ID | MK06_HUMAN |
| Accession Number | NP_002739.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | HSPVGSPLKSIQ |
| Specificity | Reported variants represent identical protein (NP_056621.4; NP_081694.1). |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites. Ubiquitination at Met-1 leads to degradation by the proteasome pathway. |
| Function | Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle. |
| Protein Name | Mitogen-Activated Protein Kinase 6Map Kinase 6Mapk 6Extracellular Signal-Regulated Kinase 3Erk-3Map Kinase Isoform P97P97-Mapk |
| Database Links | Reactome: R-HSA-5687128 |
| Cellular Localisation | CytoplasmNucleusTranslocates To The Cytoplasm Following Interaction With Mapkapk5 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 6 antibodyAnti-Map Kinase 6 antibodyAnti-Mapk 6 antibodyAnti-Extracellular Signal-Regulated Kinase 3 antibodyAnti-Erk-3 antibodyAnti-Map Kinase Isoform P97 antibodyAnti-P97-Mapk antibodyAnti-MAPK6 antibodyAnti-ERK3 antibodyAnti-PRKM6 antibody |
Information sourced from Uniprot.org