• Western blot analysis of extracts of various cell lines, using ERCC4 antibody (STJ110418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunoprecipitation analysis of 150 Mu g extracts of 293T cells using 3 Mu g ERCC4 antibody (STJ110418). Western blot was performed from the immunoprecipitate using ERCC4 antibody (STJ110418) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug ERCC4 antibody (STJ110418). Western blot was performed from the immunoprecipitate using ERCC4 antibody (STJ110418) at a dilition of 1:1000.
  • Western blot analysis of various lysates using ERCC4 Rabbit polyclonal antibody (STJ110418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ERCC4 antibody (513-782) (STJ110418)

SKU:
STJ110418

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ERCC4 (513-782) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERCC4
Gene ID: 2072
Uniprot ID: XPF_HUMAN
Immunogen Region: 513-782
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 513-782 of human ERCC4 (NP_005227.1).
Immunogen Sequence: GYRREISSSPESCPEEIKHE EFDVNLSSDAAFGILKEPLT IIHPLLGCSDPYALTRVLHE VEPRYVVLYDAELTFVRQLE IYRASRPGKPLRVYFLIYGG STEEQRYLTALRKEKEAFEK LIREKASMVVPEEREGRDET NLDLVRGTASADVSTDTRKA GGQEQNGTQQSIVVDMREFR SELPSLIHRRGIDIEPVTLE VGDYILTPEMCVERKSISDL IGSLNNGRLYSQCISMSRY
Post Translational Modifications Acetylation at Lys-911 by KAT5 promotes interaction with ERCC1 by disrupting a salt bridge between Glu-907 and Lys-911, thereby exposing a second binding site for ERCC1. Deacetylated by SIRT1.
Function Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair, and which is essential for nucleotide excision repair (NER) and interstrand cross-link (ICL) repair.
Protein Name Dna Repair Endonuclease Xpf
Dna Excision Repair Protein Ercc-4
Dna Repair Protein Complementing Xp-F Cells
Xeroderma Pigmentosum Group F-Complementing Protein
Database Links Reactome: R-HSA-5685938
Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6783310
Cellular Localisation Nucleus
Chromosome
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Dna Repair Endonuclease Xpf antibody
Anti-Dna Excision Repair Protein Ercc-4 antibody
Anti-Dna Repair Protein Complementing Xp-F Cells antibody
Anti-Xeroderma Pigmentosum Group F-Complementing Protein antibody
Anti-ERCC4 antibody
Anti-ERCC11 antibody
Anti-XPF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance