| Host: | Rabbit |
| Applications: | WB/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ERCC4 |
| Gene ID: | 2072 |
| Uniprot ID: | XPF_HUMAN |
| Immunogen Region: | 513-782 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 513-782 of human ERCC4 (NP_005227.1). |
| Immunogen Sequence: | GYRREISSSPESCPEEIKHE EFDVNLSSDAAFGILKEPLT IIHPLLGCSDPYALTRVLHE VEPRYVVLYDAELTFVRQLE IYRASRPGKPLRVYFLIYGG STEEQRYLTALRKEKEAFEK LIREKASMVVPEEREGRDET NLDLVRGTASADVSTDTRKA GGQEQNGTQQSIVVDMREFR SELPSLIHRRGIDIEPVTLE VGDYILTPEMCVERKSISDL IGSLNNGRLYSQCISMSRY |
| Post Translational Modifications | Acetylation at Lys-911 by KAT5 promotes interaction with ERCC1 by disrupting a salt bridge between Glu-907 and Lys-911, thereby exposing a second binding site for ERCC1. Deacetylated by SIRT1. |
| Function | Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair, and which is essential for nucleotide excision repair (NER) and interstrand cross-link (ICL) repair. |
| Protein Name | Dna Repair Endonuclease XpfDna Excision Repair Protein Ercc-4Dna Repair Protein Complementing Xp-F CellsXeroderma Pigmentosum Group F-Complementing Protein |
| Database Links | Reactome: R-HSA-5685938Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6783310 |
| Cellular Localisation | NucleusChromosomeLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Dna Repair Endonuclease Xpf antibodyAnti-Dna Excision Repair Protein Ercc-4 antibodyAnti-Dna Repair Protein Complementing Xp-F Cells antibodyAnti-Xeroderma Pigmentosum Group F-Complementing Protein antibodyAnti-ERCC4 antibodyAnti-ERCC11 antibodyAnti-XPF antibody |
Information sourced from Uniprot.org