| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EPRS1 |
| Gene ID: | 2058 |
| Uniprot ID: | SYEP_HUMAN |
| Immunogen Region: | 900-1000 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human EPRS (NP_004437.2). |
| Immunogen Sequence: | EAKVLFDKVASQGEVVRKLK TEKAPKDQVDIAVQELLQLK AQYKSLIGVEYKPVSATGAE DKDKKKKEKENKSEKQNKPQ KQNDGQRKDPSKNQGGGLSS S |
| Post Translational Modifications | Phosphorylated at Ser-886 by CDK5. Phosphorylated at Ser-999 by RPS6KB1.triggers EPRS1 release from the aminoacyl-tRNA synthetase multienzyme complex. In monocytes, the IFN-gamma-induced sequential phosphorylation at Ser-886 and Ser-999 releases EPRS1 from the aminoacyl-tRNA synthetase multienzyme complex, allowing its association with the GAIT complex. Phosphorylation at Ser-999 is specifically required for the RPL13A-mediated interaction of the GAIT complex with eIF4G. Phosphorylation at Ser-999 by RPS6KB1, is also induced by insulin through activation of the mTORC1 signaling pathway and promotes the interaction of EPRS1 with SLC27A1. |
| Function | Multifunctional protein which primarily functions within the aminoacyl-tRNA synthetase multienzyme complex, also known as multisynthetase complex. Within the complex it catalyzes the attachment of both L-glutamate and L-proline to their cognate tRNAs in a two-step reaction where the amino acid is first activated by ATP to form a covalent intermediate with AMP. Subsequently, the activated amino acid is transferred to the acceptor end of the cognate tRNA to form L-glutamyl-tRNA(Glu) and L-prolyl-tRNA(Pro). Upon interferon-gamma stimulation, EPRS1 undergoes phosphorylation, causing its dissociation from the aminoacyl-tRNA synthetase multienzyme complex. It is recruited to form the GAIT complex, which binds to stem loop-containing GAIT elements found in the 3'-UTR of various inflammatory mRNAs, such as ceruloplasmin. The GAIT complex inhibits the translation of these mRNAs, allowing interferon-gamma to redirect the function of EPRS1 from protein synthesis to translation inhibition in specific cell contexts. Furthermore, it can function as a downstream effector in the mTORC1 signaling pathway, by promoting the translocation of SLC27A1 from the cytoplasm to the plasma membrane where it mediates the uptake of long-chain fatty acid by adipocytes. Thereby, EPRS1 also plays a role in fat metabolism and more indirectly influences lifespan. |
| Protein Name | Bifunctional Glutamate/Proline--Trna Ligase Bifunctional Aminoacyl-Trna Synthetase Cell Proliferation-Inducing Gene 32 Protein Glutamatyl-Prolyl-Trna Synthetase Includes - Glutamate--Trna Ligase Glutamyl-Trna Synthetase Glurs - Proline--Trna Ligase Prolyl-Trna Synthetase |
| Database Links | Reactome: R-HSA-2408522 Reactome: R-HSA-379716 Reactome: R-HSA-6782315 Reactome: R-HSA-9856649 |
| Cellular Localisation | Cytoplasm Cytosol Membrane Peripheral Membrane Protein Translocates From Cytosol To Membranes Upon Phosphorylation At Ser-999 |
| Alternative Antibody Names | Anti-Bifunctional Glutamate/Proline--Trna Ligase antibody Anti-Bifunctional Aminoacyl-Trna Synthetase antibody Anti-Cell Proliferation-Inducing Gene 32 Protein antibody Anti-Glutamatyl-Prolyl-Trna Synthetase Includes - Glutamate--Trna Ligase antibody Anti-Glutamyl-Trna Synthetase antibody Anti-Glurs - Proline--Trna Ligase antibody Anti-Prolyl-Trna Synthetase antibody Anti-EPRS1 antibody Anti-EPRS antibody Anti-GLNS antibody Anti-PARS antibody Anti-QARS antibody Anti-QPRS antibody Anti-PIG32 antibody |
Information sourced from Uniprot.org