| Host: | Goat |
| Applications: | Pep-ELISA/IF/IHC/FC |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-EPHB6 (Internal) is suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary testing showed a band at approx 80kDa in U251 cell lysate and approx.75kDa in Human Pancreas lysate after 1-2µg/ml antibody staining (calculated MW of 79.8kDa according to |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | EPHB6 |
| Gene ID: | 2051 |
| Uniprot ID: | EPHB6_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_004436.2; NP_001267723.2 |
| Immunogen Sequence: | EVIAHGKHTTSSD |
| Post Translational Modifications | Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion. |
| Function | Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2. |
| Protein Name | Ephrin Type-B Receptor 6HepTyrosine-Protein Kinase-Defective Receptor Eph-6 |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928662Reactome: R-HSA-3928664Reactome: R-HSA-3928665 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinIsoform 3: Secreted |
| Alternative Antibody Names | Anti-Ephrin Type-B Receptor 6 antibodyAnti-Hep antibodyAnti-Tyrosine-Protein Kinase-Defective Receptor Eph-6 antibodyAnti-EPHB6 antibody |
Information sourced from Uniprot.org