| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EPHB6 |
| Gene ID: | 2051 |
| Uniprot ID: | EPHB6_HUMAN |
| Immunogen Region: | 615-729 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 615-729 of human EPHB6 (NP_001267724.2). |
| Immunogen Sequence: | HFDQLVAAFDKMIRKPDTLQ AGGDPGERPSQALLTPVALD FPCLDSPQAWLSAIGLECYQ DNFSKFGLCTFSDVAQLSLE DLPALGITLAGHQKKLLHHI QLLQQHLRQQGSVEV |
| Tissue Specificity | Expressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney. Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcomas and astrocytomas. Isoform 3 is not detected in normal tissues. |
| Post Translational Modifications | Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion. |
| Function | Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2. |
| Protein Name | Ephrin Type-B Receptor 6 Hep Tyrosine-Protein Kinase-Defective Receptor Eph-6 |
| Database Links | Reactome: R-HSA-2682334 Reactome: R-HSA-3928662 Reactome: R-HSA-3928664 Reactome: R-HSA-3928665 |
| Cellular Localisation | Membrane Single-Pass Type I Membrane Protein Isoform 3: Secreted |
| Alternative Antibody Names | Anti-Ephrin Type-B Receptor 6 antibody Anti-Hep antibody Anti-Tyrosine-Protein Kinase-Defective Receptor Eph-6 antibody Anti-EPHB6 antibody |
Information sourced from Uniprot.org