| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EPHB2 (250-540) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EPHB2 |
| Gene ID: |
2048 |
| Uniprot ID: |
EPHB2_HUMAN |
| Immunogen Region: |
250-540 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 250-540 of human EPHB2 (NP_059145.2). |
| Immunogen Sequence: |
PIGRCMCKAGFEAVENGTVC RGCPSGTFKANQGDEACTHC PINSRTTSEGATNCVCRNGY YRADLDPLDMPCTTIPSAPQ AVISSVNETSLMLEWTPPRD SGGREDLVYNIICKSCGSGR GACTRCGDNVQYAPRQLGLT EPRIYISDLLAHTQYTFEIQ AVNGVTDQSPFSPQFASVNI TTNQAAPSAVSIMHQVSRTV DSITLSWSQPDQPNGVILDY ELQYYEKELSEYNATAIKS |
| Tissue Specificity | Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain. |
| Post Translational Modifications | Autophosphorylated.ligand binding stimulates autophosphorylation on tyrosine residues. Polyubiquitinated.ligand binding stimulates ubiquitination. Ubiquitinated by RNF186 at Lys-891, mainly through 'Lys-27'-linked polyubiquitin chains. Ligand binding induces cleavage by matrix metalloproteinases (MMPs) such as MMP7/MMP9, producing an EphB2/N-terminal fragment (NTF) and a C-terminal long fragment (EphB2-LF). EphB2-LF is further cleaved by MMPs, producing EphB2/CTF1 which is further cleaved by the PS1/gamma-secretase producing EphB2/CTF2. |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation. |
| Protein Name | Ephrin Type-B Receptor 2Developmentally-Regulated Eph-Related Tyrosine KinaseElk-Related Tyrosine KinaseEph Tyrosine Kinase 3Eph-Like Kinase 5Ek5Hek5Renal Carcinoma Antigen Ny-Ren-47Tyrosine-Protein Kinase Tyro5Tyrosine-Protein Kinase Receptor Eph-3 Cleaved Into - Ephb2/Ctf1 - Ephb2/Ctf2 |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-373760Reactome: R-HSA-3928662Reactome: R-HSA-3928664Reactome: R-HSA-3928665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionAxonDendrite |
| Alternative Antibody Names | Anti-Ephrin Type-B Receptor 2 antibodyAnti-Developmentally-Regulated Eph-Related Tyrosine Kinase antibodyAnti-Elk-Related Tyrosine Kinase antibodyAnti-Eph Tyrosine Kinase 3 antibodyAnti-Eph-Like Kinase 5 antibodyAnti-Ek5 antibodyAnti-Hek5 antibodyAnti-Renal Carcinoma Antigen Ny-Ren-47 antibodyAnti-Tyrosine-Protein Kinase Tyro5 antibodyAnti-Tyrosine-Protein Kinase Receptor Eph-3 Cleaved Into - Ephb2/Ctf1 - Ephb2/Ctf2 antibodyAnti-EPHB2 antibodyAnti-DRT antibodyAnti-EPHT3 antibodyAnti-EPTH3 antibodyAnti-ERK antibodyAnti-HEK5 antibodyAnti-TYRO5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
