• Immunoprecipitation of EPB41 in 300 µg extracts from K-562 cells using 3 µg EPB41 Rabbit polyclonal antibody (STJ11105556). Western blot analysis was performed using EPB41 Rabbit polyclonal antibody (STJ11105556) at 1:500 dilution.
  • Western blot analysis of various lysates using EPB41 Rabbit polyclonal antibody (STJ11105556) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EPB41 antibody (700-800) (STJ11105556)

SKU:
STJ11105556

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein 4.1 (700-800) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPB41
Gene ID: 2035
Uniprot ID: EPB41_HUMAN
Immunogen Region: 700-800
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human EPB41 (NP_001159477.1).
Immunogen Sequence: RPSEWDKRLSTHSPFRTLNI NGQIPTGEGPPLVKTQTVTI SDNANAVKSEIPTKDVPIVH TETKTITYEAAQTDDNSGDL DPGVLLTAQTITSETPSSTT T
Post Translational Modifications Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions. Phosphorylation on Tyr-660 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex. O-glycosylated.contains N-acetylglucosamine side chains in the C-terminal domain.
Function Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.
Protein Name Protein 4.1
P4.1
4.1r
Band 4.1
Epb4.1
Erythrocyte Membrane Protein Band 4.1
Database Links Reactome: R-HSA-6794361
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Nucleus
Alternative Antibody Names Anti-Protein 4.1 antibody
Anti-P4.1 antibody
Anti-4.1r antibody
Anti-Band 4.1 antibody
Anti-Epb4.1 antibody
Anti-Erythrocyte Membrane Protein Band 4.1 antibody
Anti-EPB41 antibody
Anti-E41P antibody

Information sourced from Uniprot.org

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