• Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using EP300 rabbit polyclonal antibody (STJ114983) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using EP300 rabbit polyclonal antibody (STJ114983) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using EP300 rabbit polyclonal antibody (STJ114983) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using EP300 rabbit polyclonal antibody (STJ114983) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using EP300 rabbit polyclonal antibody (STJ114983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EP300 antibody (1-270) (STJ114983)

SKU:
STJ114983

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Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EP300 (1-270) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EP300
Gene ID: 2033
Uniprot ID: EP300_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EP300 (NP_001420.2).
Immunogen Sequence: MAENVVEPGPPSAKRPKLSS PALSASASDGTDFGSLFDLE HDLPDELINSTELGLTNGGD INQLQTSLGMVQDAASKHKQ LSELLRSGSSPNLNMGVGGP GQVMASQAQQSSPGLGLINS MVKSPMTQAGLTSPNMGMGT SGPNQGPTQSTGMMNSPVNQ PAMGMNTGMNAGMNPGMLAA GNGQGIMPNQVMNGSIGAGR GRQNMQYPNPGMGSAGNLLT EPLQQGSPQMGGQTGLRGP
Post Translational Modifications Acetylated on Lys at up to 17 positions by intermolecular autocatalysis. Deacetylated in the transcriptional repression domain (CRD1) by SIRT1, preferentially at Lys-1020. Deacetylated by SIRT2, preferentially at Lys-418, Lys-423, Lys-1542, Lys-1546, Lys-1549, Lys-1699, Lys-1704 and Lys-1707. Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1. Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1. Sumoylated.sumoylation in the transcriptional repression domain (CRD1) mediates transcriptional repression. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3. Probable target of ubiquitination by FBXO3, leading to rapid proteasome-dependent degradation. Phosphorylated by HIPK2 in a RUNX1-dependent manner. This phosphorylation that activates EP300 happens when RUNX1 is associated with DNA and CBFB. Phosphorylated by ROCK2 and this enhances its activity. Phosphorylation at Ser-89 by AMPK reduces interaction with nuclear receptors, such as PPARG.
Function Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of p53/TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Following DNA damage, forms a stress-responsive p53/TP53 coactivator complex with JMY which mediates p53/TP53 acetylation, thereby increasing p53/TP53-dependent transcription and apoptosis. Promotes chromatin acetylation in heat shock responsive HSP genes during the heat shock response (HSR), thereby stimulating HSR transcription. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acetylates 'Lys-247' of EGR2. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription.exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity. Acetylates XBP1 isoform 2.acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity. Acetylates PCNA.acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER). Acetylates MEF2D. Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degradation, this mechanism may be involved in CD4/CD8 lineage differentiation. Acetylates GABPB1, impairing GABPB1 heterotetramerization and activity. Acetylates PCK1 and promotes PCK1 anaplerotic activity. Acetylates RXRA and RXRG. Acetylates isoform M2 of PKM (PKM2), promoting its homodimerization and conversion into a protein kinase. Acetylates RPTOR in response to leucine, leading to activation of the mTORC1 complex. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA), 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA), lactoyl-CoA or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation, 2-hydroxyisobutyrylation, lactylation or propionylation, respectively. Acts as a histone crotonyltransferase.crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (2E)-butenoyl-CoA (crotonyl-CoA) concentration is low. Also acts as a histone butyryltransferase.butyrylation marks active promoters. Catalyzes histone lactylation in macrophages by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription. Acts as a protein-lysine 2-hydroxyisobutyryltransferase.regulates glycolysis by mediating 2-hydroxyisobutyrylation of glycolytic enzymes. Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway. (Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein.
Protein Name Histone Acetyltransferase P300
P300 Hat
E1a-Associated Protein P300
Histone Butyryltransferase P300
Histone Crotonyltransferase P300
Protein 2-Hydroxyisobutyryltransferase P300
Protein Lactyltransferas P300
Protein Propionyltransferase P300
Database Links Reactome: R-HSA-1234158
Reactome: R-HSA-1368082
Reactome: R-HSA-156711
Reactome: R-HSA-1912408
Reactome: R-HSA-1989781
Reactome: R-HSA-201722
Reactome: R-HSA-210744
Reactome: R-HSA-2122947
Reactome: R-HSA-2173796
Reactome: R-HSA-2197563
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-3134973
Reactome: R-HSA-3214847
Reactome: R-HSA-3371568
Reactome: R-HSA-381340
Reactome: R-HSA-3899300
Reactome: R-HSA-400253
Reactome: R-HSA-5250924
Reactome: R-HSA-5617472
Reactome: R-HSA-5621575
Reactome: R-HSA-5689901
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6804114
Reactome: R-HSA-6804758
Reactome: R-HSA-6804760
Reactome: R-HSA-6811555
Reactome: R-HSA-8866907
Reactome: R-HSA-8936459
Reactome: R-HSA-8939243
Reactome: R-HSA-8941856
Reactome: R-HSA-8941858
Reactome: R-HSA-8951936
Reactome: R-HSA-9013508
Reactome: R-HSA-9013695
Reactome: R-HSA-9018519
Reactome: R-HSA-9029569
Reactome: R-HSA-9031628
Reactome: R-HSA-918233
Reactome: R-HSA-933541
Reactome: R-HSA-9614657
Reactome: R-HSA-9616222
Reactome: R-HSA-9617629
Reactome: R-HSA-9701898
Reactome: R-HSA-9707616
Reactome: R-HSA-9735871
Reactome: R-HSA-9759194
Reactome: R-HSA-9793380
Reactome: R-HSA-9818027
Reactome: R-HSA-9818028
Cellular Localisation Cytoplasm
Nucleus
Chromosome
Localizes To Active Chromatin: Colocalizes With Histone H3 Acetylated And/Or Crotonylated At 'Lys-18' (H3k18ac And H3k18cr
Respectively)
In The Presence Of Alx1 Relocalizes From The Cytoplasm To The Nucleus
Colocalizes With Rock2 In The Nucleus
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Histone Acetyltransferase P300 antibody
Anti-P300 Hat antibody
Anti-E1a-Associated Protein P300 antibody
Anti-Histone Butyryltransferase P300 antibody
Anti-Histone Crotonyltransferase P300 antibody
Anti-Protein 2-Hydroxyisobutyryltransferase P300 antibody
Anti-Protein Lactyltransferas P300 antibody
Anti-Protein Propionyltransferase P300 antibody
Anti-EP300 antibody
Anti-P300 antibody

Information sourced from Uniprot.org

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