Anti-Enolase 1/2 antibody (1-434aa) [AT1G7] (STJA0041798)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
ImmunogenRecombinant human Alpha-enolase (1-434aa) purified from E. coli
General Information
| Short Description | Mouse monoclonal anti-Enolase 1/2 (1-434aa) for use in ELISA, WB, ICC, IF and FACS in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC/IF/FACS |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | AT1G7 |
| Isotype | IgG2ak |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | By protein-A affinity chromatography |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ENO1 |
| Gene ID | 2023 |
| Uniprot ID | ENOA_HUMAN |
| Accession Number | NP_001419 |
| Immunogen | Recombinant human Alpha-enolase (1-434aa) purified from E. coli |
| Immunogen Region | 1-434aa |
Additional Info
| Tissue Specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. |
| Post Translational Modifications | ISGylated. Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity. |
| Function | Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. Isoform MBP-1: Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. |
| Protein Name | Alpha-Enolase2-Phospho-D-Glycerate Hydro-LyaseC-Myc Promoter-Binding ProteinEnolase 1Mbp-1Mpb-1Non-Neural EnolaseNnePhosphopyruvate HydratasePlasminogen-Binding Protein |
| Database Links | Reactome: R-HSA-70171Reactome: R-HSA-70263Reactome: R-HSA-9636667 |
| Cellular Localisation | CytoplasmCell MembraneMyofibrilSarcomereM LineCan Translocate To The Plasma Membrane In Either The Homodimeric (Alpha/Alpha) Or Heterodimeric (Alpha/Gamma) FormEno1 Is Localized To The M LineIsoform Mbp-1: Nucleus |
| Alternative Antibody Names | Anti-Alpha-Enolase antibodyAnti-2-Phospho-D-Glycerate Hydro-Lyase antibodyAnti-C-Myc Promoter-Binding Protein antibodyAnti-Enolase 1 antibodyAnti-Mbp-1 antibodyAnti-Mpb-1 antibodyAnti-Non-Neural Enolase antibodyAnti-Nne antibodyAnti-Phosphopyruvate Hydratase antibodyAnti-Plasminogen-Binding Protein antibodyAnti-ENO1 antibodyAnti-ENO1L1 antibodyAnti-MBPB1 antibodyAnti-MPB1 antibody |
Information sourced from Uniprot.org