| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ENAH |
| Gene ID: | 55740 |
| Uniprot ID: | ENAH_HUMAN |
| Immunogen Region: | 412-591 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 412-591 of human ENAH (NP_001008493.1). |
| Immunogen Sequence: | FPSGGNAIGVNSASSKTDTG RGNGPLPLGGSGLMEEMSAL LARRRRIAEKGSTIETEQKE DKGEDSEPVTSKASSTSTPE PTRKPWERTNTMNGSKSPVI SRRDSPRKNQIVFDNRSYDS LHRPKSTPLSQPSANGVQTE GLDYDRLKQDILDEMRKELT KLKEELIDAIRQELSKSNTA |
| Tissue Specificity | Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. |
| Post Translational Modifications | NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions. |
| Function | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. |
| Protein Name | Protein Enabled Homolog |
| Database Links | Reactome: R-HSA-202433 Reactome: R-HSA-376176 |
| Cellular Localisation | Cytoplasm Cytoskeleton Cell Projection Lamellipodium Filopodium Synapse Cell Junction Focal Adhesion Targeted To The Leading Edge Of Lamellipodia And Filopodia By Mrl Family Members Colocalizes At Filopodial Tips With A Number Of Other Proteins Including Vinculin And Zyxlin Colocalizes With N-Wasp At The Leading Edge Colocalizes With Gphn And Pfn At Synapses |
| Alternative Antibody Names | Anti-Protein Enabled Homolog antibody Anti-ENAH antibody Anti-MENA antibody |
Information sourced from Uniprot.org
